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OAC1

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Chemical Structure| 300586-90-7 同义名 : BAS 00287861
CAS号 : 300586-90-7
货号 : A511757
分子式 : C14H11N3O
纯度 : 99%+
分子量 : 237.26
MDL号 : MFCD00572806
存储条件：

粉末 Keep in dark place,Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 40 mg/mL(168.59 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Oct4 is a key regulator for ESC (embryonic stem cell) pluripotency. Reduced expression of Oct4 results in differentiation of ESCs into trophectodermal cells, and ovexpression of Oct4 leads to differentiation of ESCs along the mesodermal and primitive endodermal lineages. OAC1 (Oct4-activating compound 1), was found to activate both Oct4 and Nanog promoter-driven luciferase reporter genes. By counting the number of clones with ESC-like morphology at day 18, it was found that both OAC1 enhanced the 4F (a transcription factor quartet)-induced reprogramming efficiency considerably. The number of colonies with ESC-like morphology increased two fold or more in 4F plus OAC (4F+OAC) treatment, compared with the 4F treatment alone. Furthermore, addition of OAC1 to the reprogramming mixture considerably accelerated the appearance of iPSC-like colonies. Treatment of compound OAC1 started on day 1 and lasted for 7 d. Many GFP+ single cells were seen in treated cells at day 2 and the GFP+ colonies started to appear at day 3. At day 5, a more than threefold increase in the number of GFP+ colonies was detected in OAC1-treated, 4F-transduced cells (4F+OAC1), compared with vehicle-treated, 4F-transduced cells. At day 8, an approximately fourfold increase in the number of GFP+ colonies were observed in 4F+OAC1-treated cells (2.75% reprogramming efficiency), compared with 4F-treated cells (0.68% efficiency)[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

4.21mL

0.84mL

0.42mL

21.07mL

4.21mL

2.11mL

42.15mL

8.43mL

4.21mL
参考文献

[1]Li W, Tian E, et al. Identification of Oct4-activating compounds that enhance reprogramming efficiency. Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):20853-8.

[2]Li W, Tian E, Chen ZX, et al. Identification of Oct4-activating compounds that enhance reprogramming efficiency. Proc Natl Acad Sci U S A. 2012;109(51):20853‐20858