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Crotonoside

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Chemical Structure| 1818-71-9 同义名 : 巴豆苷（异鸟苷） ;Isoguanosine;2-Hydroxyadenosine;NSC 12161;Isoguanine riboside
CAS号 : 1818-71-9
货号 : A508462
分子式 : C10H13N5O5
纯度 : 95%
分子量 : 283.241
MDL号 : MFCD04971989
存储条件：

粉末 Keep in dark place,Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 25 mg/mL(88.26 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Crotonoside is isolated from Chinese medicinal herb, Croton and inhibits FLT3 and HDAC3/6, exhibits selective inhibition in acute myeloid leukemia (AML) cells. In vivo, crotonoside treatment at 70 and 35 mg/kg/d produced significant AML tumor inhibition rates of 93.5% and 73.6%, respectively. Crotonoside (0 - 200 μM) selectively inhibits the viability of AML cell line MV4-11, MOLM-13 (with FLT3-ITD mutant) and KG-1 (without FLT3-ITD mutant) in a dose-dependent manner with an IC50 of 11.6 μM, 12.7 μM and 17.2 μM, respectively. Crotonoside (0 – 100 μM; 7 hours) inhibits the phosphorylation of FLT3. Erk1/2, Akt/mTOR and STAT5 is strongly inhibited by crotonoside at higher concentration of 12.5 μM in a concentration-dependent manner. Crotonoside (0 - 100 μM; 12 hours) exhibits a dose-dependent increase in the percentage of G0/G1 phase and a dose-dependent decrease in the percentage of G2/M and S phases cells[3].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.53mL

0.71mL

0.35mL

17.65mL

3.53mL

1.77mL

35.31mL

7.06mL

3.53mL
参考文献

[1]Yan P, Zhang L, et al. Pharmacokinetics and tissue distribution of crotonoside. Xenobiotica. 2017 Jan 19:1-9.

[2]Kim JH, Lee SJ, et al. Isolation of isoguanosine from Croton tiglium and its antitumor activity. Arch Pharm Res. 1994 Apr;17(2):115-8.

[3]Li YZ, Yu S, Yan PA, et al. Crotonoside exhibits selective post-inhibition effect in AML cells via inhibition of FLT3 and HDAC3/6. Oncotarget. 2017;8(61):103087-103099. Published 2017 Sep 8