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D-α-Hydroxyglutaric acid

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Chemical Structure| 13095-47-1 同义名 : (R)-2-Hydroxyglutarate;(R)-2-Hydroxyglutaric acid;D-2-Hydroxyglutaric Acid;D-2-HG;(R)-2-Hydroxypentanedioic acid
CAS号 : 13095-47-1
货号 : A496476
分子式 : C5H8O5
纯度 : 90%
分子量 : 148.114
MDL号 : MFCD16251406
存储条件：

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

H2O: 200 mg/mL(1350.31 mM)，配合低频超声助溶
动物实验配方：
生物活性
描述 D-α-Hydroxyglutaric acid is the major cumulative metabolite of the neurometabolic disease D-2-hydroxyglutaric aciduria. D-α-Hydroxyglutaric acid is a weak competitive antagonist of α-ketoglutarate (α-KG) and inhibits a number of α-KG-dependent dioxygenases, with a Ki value of 10.87 mM. D-α Hydroxyglutaric acid increases reactive oxygen species (ROS) production. D-α-Hydroxyglutaric acid binds and inhibits ATP synthase and inhibits mTOR signalling[1][2][3][4][5].In the presence of 50 mM D-2-HG and 100 μM α-ketoglutarate (α-KG), KDM7A partially inhibited both H3K9me2 and H3K27me2 peptides. Addition of 300 μM α-KG was able to reverse the inhibitory effect of 50 mM D-2-HG on Cryptosporidium hidradii KDM7A (CeKDM7A), suggesting that D-2-HG is a weakly competitive inhibitor of α-KG on CeKDM7A demethylases[1].D-α-Hydroxyglutaric acid is a weak inhibitor of TET hydroxylase. In the presence of 0.1 mM α-KG, the addition of 10 mM D-α-Hydroxyglutaric acid resulted in partial inhibition of TET2 (33%), whereas the addition of 50 mM D-α-Hydroxyglutaric acid resulted in greater inhibition of TET2 (83%). D-α Hydroxyglutaric acid inhibited TET1 less significantly[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

6.75mL

1.35mL

0.68mL

33.76mL

6.75mL

3.38mL

67.52mL

13.50mL

6.75mL
参考文献

[1]Xu W, et al. Oncometabolite 2-hydroxyglutarate is a competitive inhibitor of α-ketoglutarate-dependent dioxygenases. Cancer Cell. 2011 Jan 18;19(1):17-30.

[2]Fu X, et al. 2-Hydroxyglutarate Inhibits ATP Synthase and mTOR Signaling. Cell Metab. 2015 Sep 1;22(3):508-15.

[3]Martijn Kranendijk, et al. Progress in understanding 2-hydroxyglutaric acidurias. J Inherit Metab Dis. 2012 Jul;35(4):571-87.

[4]Martin Böttcher, et al. D-2-hydroxyglutarate interferes with HIF-1α stability skewing T-cell metabolism towards oxidative phosphorylation and impairing Th17 polarization. Oncoimmunology. 2018 Mar 26;7(7):e1445454.

[5]Alexandra Latini , et al. D-2-hydroxyglutaric acid induces oxidative stress in cerebral cortex of young rats. Eur J Neurosci. 2003 May;17(10):2017-22.