产品说明书
BMS-5
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同义名 : | LIMKi 3;LIM Kinase Inhibitor I |
| CAS号 : | 1338247-35-0 | |
| 货号 : | A496357 | |
| 分子式 : | C17H14Cl2F2N4OS | |
| 纯度 : | 99%+ | |
| 分子量 : | 431.287 | |
| MDL号 : | MFCD17019327 | |
| 存储条件： |
粉末 Sealed in dry,Store in freezer, under -20°C 液体 -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 35 mg/mL(81.15 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | LIMK1 and LIMK2 regulate the actin cytoskeleton by phosphorylating and inactivating the cofilin family of actin-depolymerizing factors; LIMK1 also acts to destabilize microtubules and regulates cell motility, including tumor metastasis[3]. BMS-5 is a potent LIMK inhibitor with IC50s of 7 nM and 8 nM for LIMK1 and LIMK2, respectively[3]. BMS-5 inhibits cofilin-Ser3 phosphorylation in a dose-dependent manner in Nf2ΔEx2 mouse Schwann cells (MSCs) with an IC50 of ~2 µM, and it reduces Nf2ΔEx2 MSC viability in a dose-dependent manner with an IC50 of 3.9 µM, but does not significantly reduce the viability of control Nf2flox2/flox2 MSCs at equivalent BMS-5 concentrations. At 10 µM BMS-5, Nf2ΔEx2 MSC viability is 40% compared to 83% for controls[4]. BMS-5 (20 or 200 μM/side) or vehicle is bilaterally infused into the hippocampus of rats immediately after contextual fear conditioning training. Rats are tested for memory consolidation 48 h after fear conditioning. Post hoc analysis shows that the group treated with 200 μM BMS-5 expresses lower freezing levels compared to the 20 μM and vehicle groups (P < 0.01). An additional experiment is performed in order to test if a short-term fear memory (STM) could be affected by LIMK inhibition. Animals are infused with 200 μM BMS-5 immediately after training and are tested in the same context 2 h later. No significant difference is found between both groups, BMS-5 and vehicle, in the test, suggesting that actin dynamics briefly after training is not crucial to STM expression[5]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
2.32mL 0.46mL 0.23mL |
11.59mL 2.32mL 1.16mL |
23.19mL 4.64mL 2.32mL |
| 参考文献 |
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