产品说明书
IBMX
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同义名 : | 3-异丁基-1-甲基黄嘌呤 ;3-Isobutyl-1-methylxanthine;Isobutylmethylxanthine;SC-2964;NSC 165960;1-Methyl-3-Isobutylxanthine |
| CAS号 : | 28822-58-4 | |
| 货号 : | A459882 | |
| 分子式 : | C10H14N4O2 | |
| 纯度 : | 98% | |
| 分子量 : | 222.244 | |
| MDL号 : | - | |
| 存储条件： |
粉末 Keep in dark place,Sealed in dry,Store in freezer, under -20°C 液体 -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 50 mg/mL(224.98 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO 无水乙醇: 7 mg/mL(31.5 mM)，注意：无水乙醇开封后，易挥发，也会吸收空气中的水分，导致溶解能力下降，请避免使用开封较久的乙醇 |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | Phosphodiesterases (PDEs) are enzymes that regulate the function of immune cells by hydrolyzing cyclic guanosine monophosphate/cGMP and cyclic adenosine monophosphate/cAMP. PDEs are divided into subfamilies (PDE3, PDE4, PDE5 and PDE7) which are mainly found in the respiratory tract[3]. IBMX is a broad-spectrum PDE inhibitor and its IC50 values for PDE3, 4 and 5 were 6.5 ± 1.2, 26.3 ± 3.9 and 31.7 ± 5.3 μM, respectively[4]. Human proximal tubular epithelial HK-2 cells were treated with different concentrations of IBMX for 24 h to elucidate the effect of IBMX on HIF-1 mRNA expression, IBMX reduced the mRNA levels in a dose dependant manner starting from 400 M concentration. HK-2 cells treated with or without IBMX were exposed to hypoxia for different durations, and cell viability was maintained in IBMX treated group at 24 h and 48 h, and the protective effect became significant at 48 h[5]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
4.50mL 0.90mL 0.45mL |
22.50mL 4.50mL 2.25mL |
45.00mL 9.00mL 4.50mL |
| 参考文献 |
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