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Echinocystic acid

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Chemical Structure| 510-30-5 同义名 : -
CAS号 : 510-30-5
货号 : A446246
分子式 : C30H48O4
纯度 : 97%
分子量 : 472.7
MDL号 : MFCD00017295
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(105.78 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Echinocystic acid (EA), a natural extract from plants of Gleditsia sinensis Lam, exhibits anti-inflammatory, antioxidant and analgesic activities in different diseases. EA (50 mg/kg, i.p. q.d) may provide neuroprotection via activation of the PI3K/AKT pathway[3]. EA has a protective role on vascular endothelial cells in Hhcy through decreasing plasma Hcy (hyperhomocysteinemia), and thus NF-κB and CYP1A1 (cytochrome P450 1A1) expression[4]. EA induces neurite outgrowth in a concentration dependent manner without affecting cell viability. Moreover, EA treatment increased phosphorylation of c-jun N-terminal kinase (JNK) and JNK inhibitor, SP600125, blocked the effect of EA on neurite outgrowth[5]. Administration of EA (5 or 15 mg/kg/day) for 12 weeks prevented lower levels of maximum stress and Young's modulus of femur induced by OVX (ovariectomy). EA also recovered bone metabolic biomarkers levels in OVX rats, including osteocalcin, alkaline phosphatese, deoxypyridinoline, and urinary calcium and phosphorus. EA (5 and 15 mg/kg/day) could prevent the alteration of total bone mineral density in the femur caused by OVX[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.12mL

0.42mL

0.21mL

10.58mL

2.12mL

1.06mL

21.16mL

4.23mL

2.12mL
参考文献

[1]Yang JH, Li B, et al. Echinocystic acid inhibits RANKL-induced osteoclastogenesis by regulating NF-κB and ERK signaling pathways. Biochem Biophys Res Commun. 2016 Sep 2;477(4):673-677.

[2]Ma Z, Wang Y, et al. Echinocystic Acid Inhibits IL-1β-Induced COX-2 and iNOS Expression in Human Osteoarthritis Chondrocytes. Inflammation. 2016 Apr;39(2):543-9.

[3]Chen B, Zhao Y, Li W, Hang J, Yin M, Yu H. Echinocystic acid provides a neuroprotective effect via the PI3K/AKT pathway in intracerebral haemorrhage mice. Ann Transl Med. 2020 Jan;8(1):6

[4]Huang CF, Wang WN, Sun CC, Wang YQ, Li L, Li Y, Li DJ. Echinocystic acid ameliorates hyperhomocysteinemia-induced vascular endothelial cell injury through regulating NF-κB and CYP1A1. Exp Ther Med. 2017 Nov;14(5):4174-4180

[5]Park HJ, Kwon H, Lee S, Jung JW, Ryu JH, Jang DS, Lee YC, Kim DH. Echinocystic Acid Facilitates Neurite Outgrowth in Neuroblastoma Neuro2a Cells and Enhances Spatial Memory in Aged Mice. Biol Pharm Bull. 2017;40(10):1724-1729

[6]Deng YT, Kang WB, Zhao JN, Liu G, Zhao MG. Osteoprotective Effect of Echinocystic Acid, a Triterpone Component from Eclipta prostrata, in Ovariectomy-Induced Osteoporotic Rats. PLoS One. 2015 Aug 28;10(8):e0136572