2-Nitrophenyl β-D-Galactopyranoside

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Chemical Structure| 369-07-3 同义名 : ONPG;ortho-Nitrophenyl β-D-galactopyranoside;NSC 83631;ortho-Nitrophenyl β-D-Galactoside;o-Nitrophenyl β-D-Galactoside;o-Nitrophenyl β-D-Galactopyranoside
CAS号 : 369-07-3
货号 : A444605
分子式 : C12H15NO8
纯度 : 97%
分子量 : 301.249
MDL号 : MFCD00063255
存储条件:

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月

溶解度 :

H2O: 8 mg/mL(26.56 mM),配合低频超声,并水浴加热至45℃助溶

动物实验配方:
生物活性
描述 ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity.Gal308 has high hydrolysis capacity for ONPG (100%)[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.32mL

0.66mL

0.33mL

16.60mL

3.32mL

1.66mL

33.20mL

6.64mL

3.32mL

参考文献

[1]Zhang X, et al. Metagenomic approach for the isolation of a thermostable β-galactosidase with high tolerance of galactose and glucose from soil samples of Turpan Basin. BMC Microbiol. 2013 Oct 24;13:237. doi: 10.1186/1471-2180-13-237.