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Demethylzeylasteral

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Chemical Structure| 107316-88-1 同义名 : T-96
CAS号 : 107316-88-1
货号 : A442158
分子式 : C29H36O6
纯度 : 99%+
分子量 : 480.59
MDL号 : MFCD16660658
存储条件：

粉末 Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 250 mg/mL(520.19 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Demethylzeylasteral (T-96) is a triterpene compound isolated from Tripterygium wilfordii Hook F, with anti-inflammatory, immunosuppressive and anti-tumor activities. T-96 inhibited TGF-β-induced migration and epithelial-mesenchymal transition both in vitro and in vivo. T-96 inhibited invasion of MDA-MB-231 and 4T1 cells via suppressing the canonical and non-canonical TGF-β signaling pathways[3]. T-96 significantly inhibited glioma cell growth and induced cell cycle arrest in G1 phase but did not induce apoptosis. Cell invasion and migration were dramatically suppressed after treatment with T-96[4]. Compared with the rats in the unilateral ureteral obstruction model group, demethylzeylasteral treatment markedly inhibited the increased concentrations of serum creatinine and blood urea nitrogen, urinary protein/creatinine ratio, and concentrations of high‑density lipoprotein and low‑density lipoprotein cholesterol, and prevented kidney damage. In addition, demethylzeylasteral inhibited the levels of TNF‑α andIL‑6 and suppressed the protein expression levels of COX‑2 (cyclooxygenase) and ICAM‑1 (intercellular adhesion molecule‑1) in the kidneys of the rats in the unilateral ureteral obstruction model. Demethylzeylasteral also significantly suppressed the protein expression of NF‑κB p65[5]. Demethylzeylasteral not only inhibits cell proliferation through cell cycle arrest at S phase, but also induces cell apoptosis in melanoma cells[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.08mL

0.42mL

0.21mL

10.40mL

2.08mL

1.04mL

20.81mL

4.16mL

2.08mL
参考文献

[1]Liu SL, Zhang SY, et al. Demethylzeylasteral exhibits dose-dependent inhibitory behaviour towards estradiol glucuronidation. Eur J Drug Metab Pharmacokinet. 2014 Jun;39(2):99-102.

[2]Zhao JW, Wang GH, et al. Demethylzeylasteral exhibits strong inhibition towards UDP-glucuronosyltransferase (UGT) 1A6 and 2B7. Molecules. 2012 Aug 8;17(8):9469-75.

[3]Li L, Ji Y, Fan J, et al. Demethylzeylasteral (T-96) inhibits triple-negative breast cancer invasion by blocking the canonical and non-canonical TGF-β signaling pathways. Naunyn Schmiedebergs Arch Pharmacol. 2019;392(5):593-603

[4]Zhang K, Fu G, Pan G, et al. Demethylzeylasteral inhibits glioma growth by regulating the miR-30e-5p/MYBL2 axis. Cell Death Dis. 2018;9(10):1035. Published 2018 Oct 10

[5]Wang Q, Xiao Y, Liu T, Yuan H, Li C. Demethylzeylasteral ameliorates inflammation in a rat model of unilateral ureteral obstruction through inhibiting activation of the NF‑κB pathway. Mol Med Rep. 2017;16(1):373-379

[6]Zhao Y, He J, Li J, et al. Demethylzeylasteral inhibits cell proliferation and induces apoptosis through suppressing MCL1 in melanoma cells. Cell Death Dis. 2017;8(10):e3133. Published 2017 Oct 26