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ML-9

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Chemical Structure| 105637-50-1 同义名 : ML-9 HCl;ML-9 hydrochloride
CAS号 : 105637-50-1
货号 : A439969
分子式 : C15H18Cl2N2O2S
纯度 : 99%+
分子量 : 361.287
MDL号 : MFCD00065525
存储条件：

粉末 Keep in dark place,Inert atmosphere,Room temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 85 mg/mL(235.27 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 5 mg/mL(13.84 mM)
动物实验配方：
生物活性
靶点

  • Calcium Channel
描述 The Ca2+/calmodulin-dependent myosin light-chain kinase (MLCK) pathway is considered the primary regulatory pathway for contraction of airway smooth muscle. ML-9 is a membrane-permeable organic substance that has been identified as an inhibitor of MLCK. ML-9 at a concentration of 30 or 100μM inhibited Ca2+ mobilization induced by a high concentration of K+, methacholine, and thapsigargin in guinea pig tracheal smooth muscle.[2] ML-9 at 30μM reduced the phosphorylation of Akt in lymph node carcinoma of the prostate (LNCaP) cells. Treatment with ML-9 (1 – 50μM, 2 – 24h) also dose- and time-dependently increased the expression level of LC3-II in LNCaP cells. Treatment of LNCaP cells with 30μM ML-9 for 24h significantly induced prostate cancer cell death.[1]
作用机制 ML-9 is a membrane-permeable organic substance that has been identified as an inhibitor of MLCK, Akt kinase, and stromal interaction molecule 1.[1]
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.77mL

0.55mL

0.28mL

13.84mL

2.77mL

1.38mL

27.68mL

5.54mL

2.77mL
参考文献

[1]Kondratskyi A, Yassine M, Slomianny C, Kondratska K, Gordienko D, Dewailly E, Lehen'kyi V, Skryma R, Prevarskaya N. Identification of ML-9 as a lysosomotropic agent targeting autophagy and cell death. Cell Death Dis. 2014 Apr 24;5(4):e1193. doi: 10.1038/cddis.2014.156

[2]Ito S, Kume H, Honjo H, Kodama I, Katoh H, Hayashi H, Shimokata K. ML-9, a myosin light chain kinase inhibitor, reduces intracellular Ca2+ concentration in guinea pig trachealis. Eur J Pharmacol. 2004 Feb 23;486(3):325-33. doi: 10.1016/j.ejphar.2004.01.013