产品说明书

H2DCFDA

Print
Chemical Structure| 4091-99-0 同义名 : 2',7'-二氯荧光素二乙酸酯 ;DCFH-DA;2',7'-Dichlorodihydrofluorescein diacetate;DCFH;2,7-Dichlorodihydrofluorescein diacetate
CAS号 : 4091-99-0
货号 : A439906
分子式 : C24H16Cl2O7
纯度 : 97%
分子量 : 487.286
MDL号 : MFCD00128955
存储条件:

粉末 Keep in dark place,Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月

溶解度 :

DMSO: 120 mg/mL(246.26 mM),配合低频超声助溶,注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO

无水乙醇: 20 mg/mL(41.04 mM),配合低频超声助溶,注意:无水乙醇开封后,易挥发,也会吸收空气中的水分,导致溶解能力下降,请避免使用开封较久的乙醇

动物实验配方:
生物活性
描述 H2DCFDA (DCFH-DA) is a probe permeable to cells and is employed for the detection of intracellular reactive oxygen species (ROS), with an excitation and emission wavelength of 488/525 nm respectively[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.05mL

0.41mL

0.21mL

10.26mL

2.05mL

1.03mL

20.52mL

4.10mL

2.05mL

参考文献

[1]Lyublinskaya OG, et al. Redox environment in stem and differentiated cells: A quantitative approach. Redox Biol. 2017 Aug;12:758-769.