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Purpurin

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Chemical Structure| 81-54-9 同义名 : Hydroxylizaric Acid;Verantin;NSC 10447
CAS号 : 81-54-9
货号 : A418868
分子式 : C14H8O5
纯度 : 95%
分子量 : 256.21
MDL号 : MFCD00001203
存储条件：

粉末 Inert atmosphere,Room Temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 120 mg/mL(468.37 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Purpurin is a natural anthraquinone compound from Rubia tinctorum L. Purpurin (Orally; 2, 6, 18 mg/kg for 3 weeks) exerts dose-dependently antidepressant-like effects on behavior and stress axis reactivity in adult male C57BL/6J mice (6-7 weeks old upon arrival) [3]. In vitro enzymatic analyses demonstrated that purpurin inhibited A-LAP (Adipocyte-derived leucine aminopeptidase) activity in a non-competitive manner with a Ki value of 20 M. In angiogenesis assays, purpurin inhibited the vascular endothelial growth factor (VEGF)-induced invasion and tube formation of human umbilical vein endothelial cells (HUVEC) [4]. Purpurin was non-toxic when fed to Drosophila and it significantly ameliorated the AD-related neurotoxic symptoms of transgenic flies expressing WT-FL (full-length) human Tau (hTau) plausibly by inhibiting Tau accumulation and reducing Tau phosphorylation. Purpurin also reduced hTau accumulation in cell culture overexpressing hTau. Importantly, Purpurin efficiently crossed an in vitro human blood-brain barrier model[5]. The administration of purpurin at 50 and 100 μM in 3T3-L1 cells, and at 40 and 80 mg/kg in mice proved to be a sensitive range: the lower concentrations affected several measured parameters, whereas at the higher doses purpurin consistently mitigated biomarkers associated with adipogenesis, and weight gain in mice[6]. Moreover, purpurin (36 μM) exposed to HCT-116 cells and incubated for 24 and 48 h could induce reactive oxygen species production, subsequently alter mitochondrion membrane, and increase the apoptotic cells in HCT-116. And purpurin induces messenger RNA expression of apoptotic genes (Bax, CASP-9, and CASP-3) in HCT-116 cells[7].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.90mL

0.78mL

0.39mL

19.52mL

3.90mL

1.95mL

39.03mL

7.81mL

3.90mL
参考文献

[1]Ranjitha S, Rajarajan G, et al. Structural and optical properties of Purpurin for dye-sensitized solar cells. Spectrochim Acta A Mol Biomol Spectrosc. 2015 Oct 5;149:997-1008.

[2]Park H, Shim JS, et al. Purpurin inhibits adipocyte-derived leucine aminopeptidase and angiogenesis in a zebrafish model. Biochem Biophys Res Commun. 2014 Jul 18;450(1):561-7.

[3]Ma L, Hu P, Zhang J, Cui W, Zhao X. Purpurin exerted antidepressant-like effects on behavior and stress axis reactivity: evidence of serotonergic engagement. Psychopharmacology (Berl). 2020 Mar;237(3):887-899

[4]Park H, Shim JS, Kim BS, Jung HJ, Huh TL, Kwon HJ. Purpurin inhibits adipocyte-derived leucine aminopeptidase and angiogenesis in a zebrafish model. Biochem Biophys Res Commun. 2014 Jul 18;450(1):561-7

[5]Viswanathan GK, Shwartz D, Losev Y, Arad E, Shemesh C, Pichinuk E, Engel H, Raveh A, Jelinek R, Cooper I, Gosselet F, Gazit E, Segal D. Purpurin modulates Tau-derived VQIVYK fibrillization and ameliorates Alzheimer's disease-like symptoms in animal model. Cell Mol Life Sci. 2020 Jul;77(14):2795-2813

[6]Nam W, Nam SH, Kim SP, Levin C, Friedman M. Anti-adipogenic and anti-obesity activities of purpurin in 3T3-L1 preadipocyte cells and in mice fed a high-fat diet. BMC Complement Altern Med. 2019 Dec 11;19(1):364

[7]Li Z, Zhou X, Zhu H, Song X, Gao H, Niu Z, Lu J. Purpurin binding interacts with LHPP protein that inhibits PI3K/AKT phosphorylation and induces apoptosis in colon cancer cells HCT-116. J Biochem Mol Toxicol. 2021 Mar;35(3):e22665