产品说明书
3,4-Dihydroxybenzylamine HBr
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同义名 : | 3,4-Dihydroxybenzylamine hydrobromide;NSC 263475 hydrobromide |
| CAS号 : | 16290-26-9 | |
| 货号 : | A399015 | |
| 分子式 : | C7H10BrNO2 | |
| 纯度 : | 97% | |
| 分子量 : | 220.064 | |
| MDL号 : | MFCD00012859 | |
| 存储条件： |
粉末 Inert atmosphere,Room Temperature 液体 -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 85 mg/mL(386.25 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | 3,4-Dihydroxybenzylamine hydrobromide (NSC 263475 hydrobromide) is an improved dopamine analog cytotoxic and inhibits DNA polymerase activity in melanoma cells. Inhibition of DNA polymerase by activated 3,4-DHBA was shown by dialysis and kinetic studies to involve an irreversible reaction which occurs at two inhibitor interaction sites as determined by a Hill plot analysis. Double-stranded DNA protected the enzyme from inhibition by 3,4-DHBA, suggesting that the inhibitory sites are at or near the template-initiator binding site[4]. 3,4-Dihydroxybenzylamine hydrobromide (NSC 263475 hydrobromide) displays growth inhibitory activity in melanoma cell lines with varying degrees of tyrosinase activity[5].3,4-Dihydroxybenzylamine (DHBA), a dopamine analog, was much less toxic than dopamine when tested against the B16 melanoma in vivo and in vitro. Daily doses of 1,000 mg DHBA/kg were better tolerated than doses of 400 mg dopamine/kg. When tested against the B16 melanoma in (C57BL/6 x DBA/2)F1 mice, DHBA had a significantly improved therapeutic effect as shown by a life-span increased 70% as compared to 48% with dopamine. DHBA shared the catecholamine property of selectively inhibiting thymidine incorporation as compared to leucine or uridine incorporation[6]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
4.54mL 0.91mL 0.45mL |
22.72mL 4.54mL 2.27mL |
45.44mL 9.09mL 4.54mL |
| 参考文献 |
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