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Gynostemma Extract

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Chemical Structure| 80321-63-7 同义名 : 七叶胆皂甙 ;Gypenoside IX;Ginsenoside C-Mx1;Notoginsenoside Fd;Gypenoside;Gynostemma Pentaphyllum
CAS号 : 80321-63-7
货号 : A371506
分子式 : C47H80O17
纯度 : 98+%
分子量 : 917.128
MDL号 : MFCD10566702
存储条件：

粉末 2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(114.49 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Gynostemma extract, a natural product isolated and purified from the herb of Gynostemma pentaphyllum (Thunb.) Makino., induces apoptosis in human hepatoma cells through the up-regulation of Bax and Bak, and down-regulation of Bcl-2, release of mitochondrial cytochrome c and activation of caspase cascade, induces ER stress and production of reactive oxygen species and Ca 2+ , changes the ratio of Bcl-2 and Bax, followed by the dysfunction of mitochondria, and causes cytochrome c release, activation of caspase-3 before leading to apoptosis. Gypenosides can inhibit invasion and migration of human tongue SCC4 cells by down-regulating proteins associated with these processes, resulting in reduced metastasis.
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.09mL

0.22mL

0.11mL

5.45mL

1.09mL

0.55mL

10.90mL

2.18mL

1.09mL
参考文献

[1]Chen JC, Lu KW, et al. Gypenosides induced G0/G1 arrest via CHk2 and apoptosis through endoplasmic reticulum stress and mitochondria-dependent pathways in human tongue cancer SCC-4 cells. Oral Oncol. 2009 Mar;45(3):273-83.

[2]Wang QF, Chen JC, et al. Regulation of Bcl-2 family molecules and activation of caspase cascade involved in gypenosides-induced apoptosis in human hepatoma cells. Cancer Lett. 2002 Sep 26;183(2):169-78.