生物活性 | |||
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描述 | Sulcotrione, a β-triketene herbicide, inhibits HPPD. Sulcotrione is a time-dependent reversible inhibitor. Previously, similar results have been seen with natural β-triketone and synthetic β-triketone NTBC. However, this is the first time that such behaviour has been observed using purified HPPD and synthetic β-triketone, namely sulcotrione. Inhibition kinetics analyses using 3 hydroxyphenylpyruvate (HPP) and sulcotrione concentrations showed that Kmapp increased with increasing sulcotrione concentration. This behaviour is consistent with data in the literature describing Sulcotrione as a competitive inhibitor of HPPD[1]. |
实验方案 | |||
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1mg | 5mg | 10mg | |
1 mM 5 mM 10 mM |
3.04mL 0.61mL 0.30mL |
15.21mL 3.04mL 1.52mL |
30.42mL 6.08mL 3.04mL |
参考文献 |
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