产品说明书
DAPI 2HCl
 |
同义名 : | 4',6-Diamidino-2-phenylindole dihydrochloride;DAPI (hydrochloride);FxCycle Violet;4',6-Diamidino-2-phenylindole |
| CAS号 : | 28718-90-3 | |
| 货号 : | A352479 | |
| 分子式 : | C16H17Cl2N5 | |
| 纯度 : | 98% | |
| 分子量 : | 350.246 | |
| MDL号 : | MFCD00012681 | |
| 存储条件： |
粉末 Inert atmosphere,Store in freezer, under -20°C 液体 -20°C:3-6个月-80°C:12个月 |
|
| 溶解度 : |
DMSO: 80 mg/mL(228.41 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO H2O: 5.5 mg/mL(15.7 mM)，配合低频超声，并水浴加热至45℃助溶 |
|
| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | DAPI dihydrochloride, a variant of DAPI dye, exhibits strong affinity for DNA, specifically binding to the AT base pairs within the minor groove of double-stranded DNA, where a single DAPI molecule occupies three base pair positions. This binding significantly amplifies DAPI's fluorescence intensity, enabling its use in live and fixed cell staining observable via fluorescence microscopy. The fluorescent intensity is leveraged to quantify DNA, with DAPI's ability to penetrate intact cell membranes facilitating comprehensive cellular staining[1]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
|
1 mM 5 mM 10 mM |
2.86mL 0.57mL 0.29mL |
14.28mL 2.86mL 1.43mL |
28.55mL 5.71mL 2.86mL |
| 参考文献 |
|---|