Bisbenzimide H 33258

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Chemical Structure| 23491-45-4 同义名 : bisBenzimide H 33258 trihydrochloride;H 33258 trihydrochloride;Hoechst 33378;H33378;HOE33378;Pibenzimol;NSC 322921;HOE 33258;Bisbenzimide;Hoechst 33258;Hoechst 33258 trihydrochloride
CAS号 : 23491-45-4
货号 : A346521
分子式 : C25H27Cl3N6O
纯度 : 99%+
分子量 : 533.88
MDL号 : MFCD00012679
存储条件：

Pure form Inert atmosphere,2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(196.67 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 12 mg/mL(22.48 mM)，配合低频超声，并水浴加热至45℃助溶
动物实验配方：
生物活性
描述 Hoechst 33258 is a nuclear marker dye in Hoechst series, primarily binding to the A/T-rich regions of DNA strands. Its binding affinity is not restricted to DNA, but the fluorescence intensity is significantly enhanced when bound to A/T-rich double-stranded DNA, making it a valuable tool for live cell labeling. The fluorescence intensity of Hoechst dye increases with the solution's pH, enabling its application across a range of biological research fields[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.87mL

0.37mL

0.19mL

9.37mL

1.87mL

0.94mL

18.73mL

3.75mL

1.87mL
参考文献

[1]Wang XJ, et al. Newly synthesized bis-benzimidazole derivatives exerting anti-tumor activity through inductionof apoptosis and autophagy. Bioorg Med Chem Lett. 2012 Oct 1;22(19):6297-300.