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o-Phenanthroline

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Chemical Structure| 66-71-7 同义名 : 1,10-菲罗啉(无水) ;1,10-Phenanthroline
CAS号 : 66-71-7
货号 : A326753
分子式 : C12H8N2
纯度 : 98%
分子量 : 180.21
MDL号 : MFCD00011678
存储条件:

粉末 Inert atmosphere,Room Temperature

液体 -20°C:3-6个月-80°C:12个月

溶解度 :

DMSO: 105 mg/mL(582.65 mM),配合低频超声助溶,注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO

H2O: 2 mg/mL(11.1 mM),配合低频超声助溶

无水乙醇: 50 mg/mL(277.45 mM),配合低频超声助溶,注意:无水乙醇开封后,易挥发,也会吸收空气中的水分,导致溶解能力下降,请避免使用开封较久的乙醇

动物实验配方:
生物活性
描述 O-phenanthroline is a non-specific matrix metalloproteinase (MMP) inhibitor. O-phenanthroline reduced the expression of MMP3 and MMP13 mRNA levels during chondrogenic differentiation of human chondrocytes (hChs), as well as after TNFα/IL-1β exposure in an explant model. Interestingly, O-phenanthroline significantly inhibited matrix degradation in a TNFα/IL-1β-dependent model of cartilage degeneration when compared to control and natural hypoxia (2.5% O2 ). Additionally, O-phenanthroline alleviated MMP-induced cartilage degradation without affecting chondrogenesis in the explant culture[2]. The lethal effect could be explained by the kinetics of reaction of o-phenanthroline with intracellular Fe2+, i.e., the mono and bis complexes are more reactive than intracellular Fe2+[3]. H2O2 is able to induce mutation at the HGPRT locus in V79 cells and morphological transformation of C3H/10T1/2 cells. O-Phenanthroline abolishes both effects, indicating that hydroxyl radical is directly involved in mutation and carcinogenesis[4].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

5.55mL

1.11mL

0.55mL

27.75mL

5.55mL

2.77mL

55.49mL

11.10mL

5.55mL

参考文献

[1]Guan NanMu, et al. Synergistic inhibition between o-phenanthroline and chloride ion on cold rolled steel corrosion in phosphoric acid. Materials Chemistry and Physics Volume 86, Issue 1, 15 July 2004, Pages 59-68.

[2]Georgi N, Landman EB, Klein TJ, van Blitterswijk CA, Karperien M. O-Phenanthroline as modulator of the hypoxic and catabolic response in cartilage tissue-engineering models. J Tissue Eng Regen Med. 2017;11(3):724‐732

[3]Asad NR, Asad LM, Almeida CE, Leitão AC. Lethal interaction between hydrogen peroxide and o-phenanthroline in Escherichia coli. Braz J Med Biol Res. 1994;27(11):2551‐2555

[4]Nassi-Calò L, Mello-Filho C, Meneghini R. o-phenanthroline protects mammalian cells from hydrogen peroxide-induced gene mutation and morphological transformation. Carcinogenesis. 1989;10(6):1055‐1057