产品说明书
o-Phenanthroline
 |
同义名 : | 1,10-菲罗啉(无水) ;1,10-Phenanthroline |
| CAS号 : | 66-71-7 | |
| 货号 : | A326753 | |
| 分子式 : | C12H8N2 | |
| 纯度 : | 98% | |
| 分子量 : | 180.21 | |
| MDL号 : | MFCD00011678 | |
| 存储条件： |
粉末 Inert atmosphere,Room Temperature 液体 -20°C:3-6个月-80°C:12个月 |
|
| 溶解度 : |
DMSO: 105 mg/mL(582.65 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO H2O: 2 mg/mL(11.1 mM)，配合低频超声助溶 无水乙醇: 50 mg/mL(277.45 mM)，配合低频超声助溶，注意：无水乙醇开封后，易挥发，也会吸收空气中的水分，导致溶解能力下降，请避免使用开封较久的乙醇 |
|
| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | O-phenanthroline is a non-specific matrix metalloproteinase (MMP) inhibitor. O-phenanthroline reduced the expression of MMP3 and MMP13 mRNA levels during chondrogenic differentiation of human chondrocytes (hChs), as well as after TNFα/IL-1β exposure in an explant model. Interestingly, O-phenanthroline significantly inhibited matrix degradation in a TNFα/IL-1β-dependent model of cartilage degeneration when compared to control and natural hypoxia (2.5% O2 ). Additionally, O-phenanthroline alleviated MMP-induced cartilage degradation without affecting chondrogenesis in the explant culture[2]. The lethal effect could be explained by the kinetics of reaction of o-phenanthroline with intracellular Fe2+, i.e., the mono and bis complexes are more reactive than intracellular Fe2+[3]. H2O2 is able to induce mutation at the HGPRT locus in V79 cells and morphological transformation of C3H/10T1/2 cells. O-Phenanthroline abolishes both effects, indicating that hydroxyl radical is directly involved in mutation and carcinogenesis[4]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
|
1 mM 5 mM 10 mM |
5.55mL 1.11mL 0.55mL |
27.75mL 5.55mL 2.77mL |
55.49mL 11.10mL 5.55mL |
| 参考文献 |
|---|