DiR

产品说明书

	同义名 :	Cy7 DiC18; 1,1-Dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine (iodide)
	CAS号 :	100068-60-8
	货号 :	A320307
	分子式 :	C₆₃H₁₀₁IN₂
	纯度 :	98%+
	分子量 :	1013.39
	MDL号 :	MFCD03427788
	存储条件：	Pure form Keep in dark place, inert atmosphere, 2-8°C In solvent -20°C:3-6个月-80°C:12个月
	溶解度 :	DMSO: 25 mg/mL(24.67 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
	动物实验配方：

生物活性
描述	DiR, a long-chain carbocyanine dye, is part of the family of carbocyanine dyes extensively used for labeling cells, organelles, liposomes, viruses, and lipoproteins. Carbocyanine dyes, including DiO (DiOC18(3)), DiI (DiIC18(3)), DiD (DiIC18(5)), DiR, and the dialkyl aminostyryl dye DiA (4-Di-16-ASP), are employed for marking membranes and other hydrophobic structures. These dyes possess high extinction coefficients, display environmental-dependent fluorescence, and exhibit short excited-state lifetimes in lipid environments, becoming fluorescent and photostable when integrated into membranes or attached to lipophilic biomolecules^[1]. DiO, DiI, DiD, and DiR provide green, orange, red, and infrared fluorescence, respectively, enabling multicolor imaging and flow cytometric analysis of live cells. DiO and DiI are compatible with standard FITC and TRITC filters, respectively. DiI and its analogs, known for their minimal cell toxicity, are frequently used for labeling lipoproteins such as LDL and HDL. DiA is commonly utilized in neuronal tracing^[2].

生物活性

描述

DiR, a long-chain carbocyanine dye, is part of the family of carbocyanine dyes extensively used for labeling cells, organelles, liposomes, viruses, and lipoproteins.

Carbocyanine dyes, including DiO (DiOC18(3)), DiI (DiIC18(3)), DiD (DiIC18(5)), DiR, and the dialkyl aminostyryl dye DiA (4-Di-16-ASP), are employed for marking membranes and other hydrophobic structures. These dyes possess high extinction coefficients, display environmental-dependent fluorescence, and exhibit short excited-state lifetimes in lipid environments, becoming fluorescent and photostable when integrated into membranes or attached to lipophilic biomolecules^[1].

DiO, DiI, DiD, and DiR provide green, orange, red, and infrared fluorescence, respectively, enabling multicolor imaging and flow cytometric analysis of live cells. DiO and DiI are compatible with standard FITC and TRITC filters, respectively. DiI and its analogs, known for their minimal cell toxicity, are frequently used for labeling lipoproteins such as LDL and HDL. DiA is commonly utilized in neuronal tracing^[2].

实验方案
	1mg	5mg	10mg
1 mM 5 mM 10 mM	0.99mL 0.20mL 0.10mL	4.93mL 0.99mL 0.49mL	9.87mL 1.97mL 0.99mL

实验方案

1mg

5mg

10mg

1 mM

5 mM

10 mM

0.99mL

0.20mL

0.10mL

4.93mL

0.99mL

0.49mL

9.87mL

1.97mL

0.99mL

参考文献
[1]Gan WB, et al. Multicolor "DiOlistic" labeling of the nervous system using lipophilic dye combinations. Neuron. 2000 Aug;27(2):219-25. [2]Swift MJ, et al. Applied electric fields accelerate the diffusion rate and increase the diffusion distance of DiI in fixed tissue. J Neurosci Methods. 2005 Jan 30;141(1):155-63.

参考文献

[1]Gan WB, et al. Multicolor "DiOlistic" labeling of the nervous system using lipophilic dye combinations. Neuron. 2000 Aug;27(2):219-25.

[2]Swift MJ, et al. Applied electric fields accelerate the diffusion rate and increase the diffusion distance of DiI in fixed tissue. J Neurosci Methods. 2005 Jan 30;141(1):155-63.

靶点

靶点	数值