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(±)-10-Hydroxycamptothecin

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Chemical Structure| 64439-81-2 同义名 : (±)-10-HCPT;Hydroxycamptothecine;10-Hydroxycamptothecin
CAS号 : 64439-81-2
货号 : A314756
分子式 : C20H16N2O5
纯度 : 99%+
分子量 : 364.351
MDL号 : MFCD09027971
存储条件：

粉末 Keep in dark place,Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 25 mg/mL(68.62 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 10-Hydroxycamptothecin (HCPT) is an inhibitor of topoisomerase I and can affect phosphorylation of histone H1 and H3 with anticancer activity. It can be isolated from camptotheca acuminate. Gel electrophoresis found that HCPT had a selectively inhibitory effect on the phosphorylation of histone H1 and H3, but less effect on the other kinds of histones. In vivo, HCPT also exhibited a suppressive effect on histone H1 and H3 phosphorylation[1]. After treatment with HCPT at differentiation-inducing concentrations 5-20 mg/L for 6 d, Hep G2 cells were mainly arrested at G2/M phase and the PCNA expression rate was lower than that of control cells. When Hep G2 cells grew in a medium containing HCPT 5 mg/L for 6 d, the p53 expression level markedly increased in comparison with the control cells. The telomerase activity did not change in Hep G2 cells treated with HCPT 5-20 mg/L for 8 d[2]. HCPT exhibited adverse effects on mouse oocyte maturation and quality, and MT (Melatonin) administration alleviated the negative influence of HCPT[3].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.74mL

0.55mL

0.27mL

13.72mL

2.74mL

1.37mL

27.45mL

5.49mL

2.74mL
参考文献

[1]Ling YH, Xu B. Inhibition of phosphorylation of histone H1 and H3 induced by 10-hydroxycamptothecin, DNA topoisomerase I inhibitor, in murine ascites hepatoma cells. Zhongguo Yao Li Xue Bao. 1993 Nov;14(6):546-50

[2]Zhang XW, Jiang JF, Xu B. Differentiation-inducing action of 10-hydroxycamptothecin on human hepatoma Hep G2 cells. Acta Pharmacol Sin. 2000 Apr;21(4):364-8

[3]Wang L, Zhang J, Zhao C, Jia Z, Feng X. Melatonin Reverses 10-Hydroxycamptothecin-Induced Apoptosis and Autophagy in Mouse Oocyte. Reprod Sci. 2021 Jul;28(7):1839-1849