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Bruceine D

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Chemical Structure| 21499-66-1 同义名 : 鸦胆子素D
CAS号 : 21499-66-1
货号 : A313823
分子式 : C20H26O9
纯度 : 95%
分子量 : 410.415
MDL号 : MFCD00221753
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(255.84 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 5 mg/mL(12.18 mM)，配合低频超声，并水浴加热至45℃助溶
动物实验配方：
生物活性
描述 Bruceine D is an effective botanical insect antifeedant with outstanding systemic properties, causing potent pest growth inhibitory activity[3]. Bruceine D is a Notch inhibitor with anti-cancer activity and induces apoptosis in several human cancer cells. BD significantly inhibited liver tumor growth and enhanced the therapeutic effects of sorafenib in various murine HCC (Hepatocellular carcinoma) models[4]. Bruceine D exhibited potent cytotoxicity to K562 cells with IC50 of 6.37 ± 0.39 μM. In addition, bruceine D inhibited the phosphorylation of AKT and ERK. It's indicative that the potent anticancer activity of bruceine D be related to MAPK and PI3K pathways[5]. Bruceine D markedly inhibited the proliferation of wild‑type NSCLC (non‑small‑cell lung cancer) cells and epidermal growth factor receptor‑mutant cells in a dose‑ and time‑dependent manner, and significantly decreased the colony‑forming ability and migration of A549 cells[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.44mL

0.49mL

0.24mL

12.18mL

2.44mL

1.22mL

24.37mL

4.87mL

2.44mL
参考文献

[1]Liu L, Lin ZX, et al. Involvement of the mitochondrial pathway in bruceine D-induced apoptosis in Capan-2 human pancreatic adenocarcinoma cells. Int J Mol Med. 2012 Jul;30(1):93-9.

[2]Wang Y, Wu ZF, et al. In vivo anthelmintic activity of bruceine A and bruceine D from Brucea javanica against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus). Vet Parasitol. 2011 Apr 19;177(1-2):127-33.

[3]Mao G, Tian Y, Sun Z, Ou J, Xu H. Bruceine D Isolated from Brucea Javanica (L.) Merr. as a Systemic Feeding Deterrent for Three Major Lepidopteran Pests. J Agric Food Chem. 2019;67(15):4232-4239

[4]Cheng Z, Yuan X, Qu Y, et al. Bruceine D inhibits hepatocellular carcinoma growth by targeting β-catenin/jagged1 pathways. Cancer Lett. 2017;403:195-205

[5]Zhang JY, Lin MT, Tung HY, et al. Bruceine D induces apoptosis in human chronic myeloid leukemia K562 cells via mitochondrial pathway. Am J Cancer Res. 2016;6(4):819-826. Published 2016 Mar 15

[6]Xie JH, Lai ZQ, Zheng XH, et al. Apoptosis induced by bruceine D in human non‑small‑cell lung cancer cells involves mitochondrial ROS‑mediated death signaling. Int J Mol Med. 2019;44(6):2015-2026