产品说明书
L-Ascorbic acid 2-phosphate trisodium
 |
同义名 : | 2-Phospho-L-ascorbic acid trisodium |
| CAS号 : | 66170-10-3 | |
| 货号 : | A312337 | |
| 分子式 : | C6H6Na3O9P | |
| 纯度 : | 97% | |
| 分子量 : | 322.05 | |
| MDL号 : | MFCD04038081 | |
| 存储条件： |
粉末 Inert atmosphere,Room Temperature 液体 -20°C:3-6个月-80°C:12个月 |
|
| 溶解度 : |
DMSO: 5 mg/mL(15.53 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO H2O: 150 mg/mL(465.77 mM)，配合低频超声助溶 |
|
| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | L-Ascorbic acid 2-phosphate trisodium is a long-acting vitamin C derivative that stimulates collagen formation and expression[1].L-Ascorbic acid 2-phosphate trisodium can be used as a medium supplement for osteogenic differentiation of human adipose stem cells (hASCs). During osteogenic differentiation, L-Ascorbic acid 2-phosphate trisodium increased alkaline phosphatase (ALP) activity and runx2A expression in human adipose stem cells[2][3].L-Ascorbic acid 2-phosphate trisodium can significantly stimulate cell growth by adding L-Ascorbic acid 2-phosphate trisodium to the culture medium at a concentration of 0.1-1.5 mM for 2 to 3 weeks, changing the culture medium every 2 to 3 days, whereas the addition of l-ascorbic acid (Asc) could only achieve weak growth stimulation. The combination of Asc-2P and bFGF significantly promoted cell growth, but supplementation with EGF and/or insulin did not have any additional effect [1]. L-Ascorbic acid 2-phosphate trisodium is required for the efficient osteogenic differentiation of human adipose stem cells (hASCs) and acts at concentrations of 50 µM-250 µM, with higher concentrations of AsA2-P leading to increased runx2 expression and ALP activity. 150 µM AsA2-P and 10 nM concentrations of dexamethasone and 250 µM AsA2-P and 5 nM concentration of dexamethasone resulted in the highest proliferation, ALP activity and runx2 expression[3]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
|
1 mM 5 mM 10 mM |
3.11mL 0.62mL 0.31mL |
15.53mL 3.11mL 1.55mL |
31.05mL 6.21mL 3.11mL |
| 参考文献 |
|---|