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Digitonin

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Chemical Structure| 11024-24-1 同义名 : 毛地黃皂苷 ;Digitin;NSC 237417;NSC 23471
CAS号 : 11024-24-1
货号 : A300453
分子式 : C56H92O29
纯度 : 50%
分子量 : 1229.312
MDL号 : MFCD00077729
存储条件：

粉末 Sealed in dry,Room Temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(85.41 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Digitonin, a glycoside obtained from Digitalis purpurea, could increase cell permeability by binding to cholesterol molecules and reduce tumor growth. Digitonin enhanced the tumour-growth-retarding effect of CBDCA (carboplatin) given intra-arterially at 5 mg/kg but not at 25 mg/kg. Pretreatment with digitonin increases the tumour uptake of CBDCA and potentiates the cytotoxic effect of CBDCA[1]. Digitonin is thus useful both for selectively permeabilizing the plasma membrane and for intensifying the appearance of intracellular structures such as microfilaments that are normally difficult to observe in cells such as hepatocytes[2]. Perfusing a rat liver with digitonin in the concentration range 2-20 mg/ml results in complete decolorization of the organ within 45-250s[3]. The activity of rat liver microsomal squalene epoxidase is inhibited effectively by digitonin. The inhibition of squalene epoxidase by digitonin is concentration-dependent and takes place rapidly within 5 min of exposure of the microsomes to digitonin. Only 40% inhibition of cyclase activity is observed at 1 mg/ml of digitonin[4].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

0.81mL

0.16mL

0.08mL

4.07mL

0.81mL

0.41mL

8.13mL

1.63mL

0.81mL
参考文献

[1]Orczyk M, Wojciechowski K, Brezesinski G. Disordering Effects of Digitonin on Phospholipid Monolayers. Langmuir. 2017 Apr 18;33(15):3871-3881.

[2]Wojciechowski K, Orczyk M, et al. On the Interaction between Digitonin and Cholesterol in Langmuir Monolayers. Langmuir. 2016 Sep 6;32(35):9064-73.

[3]Lindnér PG, Heath D, Howell SB, Naredi PL, Hafström LR. Digitonin enhances the efficacy of carboplatin in liver tumour after intra-arterial administration. Cancer Chemother Pharmacol. 1997;40(5):444-8

[4]Fiskum G, Craig SW, Decker GL, Lehninger AL. The cytoskeleton of digitonin-treated rat hepatocytes. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3430-4

[5]Quistorff B, Grunnet N, Cornell NW. Digitonin perfusion of rat liver. A new approach in the study of intra-acinar and intracellular compartmentation in the liver. Biochem J. 1985 Feb 15;226(1):289-97

[6]Eilenberg H, Klinger E, Przedecki F, Shechter I. Inactivation and activation of various membranal enzymes of the cholesterol biosynthetic pathway by digitonin. J Lipid Res. 1989 Aug;30(8):1127-35