产品说明书

NBD-F

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Chemical Structure| 29270-56-2 同义名 : 4-Fluoro-7-nitrobenzofurazan
CAS号 : 29270-56-2
货号 : A297151
分子式 : C6H2FN3O3
纯度 : 95%
分子量 : 183.097
MDL号 : MFCD00010196
存储条件:

粉末 Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月

溶解度 :

DMSO: 105 mg/mL(573.47 mM),配合低频超声助溶,注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO

动物实验配方:
生物活性
描述 NBD-F (4-Fluoro-7-nitrobenzofurazan) is a pro-fluorescent reagent crafted for amino acid analysis, reacting with primary or secondary amines to form a fluorescent product, applicable for analyzing amino acids and low molecular weight amines[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

5.46mL

1.09mL

0.55mL

27.31mL

5.46mL

2.73mL

54.62mL

10.92mL

5.46mL

参考文献

[1]Wu X, et al. Determination of amino acid neurotransmitters in rat hippocampi by HPLC-UV using NBD-F as a derivative. Biomed Chromatogr. 2014 Apr;28(4):459-62.

[2]Ishikawa T, et al. Development of an LC-MS/MS method for the analysis of free sphingoid bases using 4-fluoro-7-nitrobenzofurazan (NBD-F). Lipids. 2014 Mar;49(3):295-304.