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D-Fructose

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Chemical Structure| 57-48-7 同义名 : D-(-)-果糖 ;D(-​)​-​Fructose;Fruit sugar;D-arabino-hexulose;D-fructofuranose;fructosteril;levulose;D-(-)-Fructose;Fructose
CAS号 : 57-48-7
货号 : A293834
分子式 : C6H12O6
纯度 : 98%
分子量 : 180.156
MDL号 : MFCD00148910
存储条件:

粉末 Inert atmosphere,Room Temperature

液体 -20°C:3-6个月-80°C:12个月

溶解度 :

H2O: 100 mg/mL(555.07 mM),配合低频超声助溶

动物实验配方:
生物活性
描述 E-Fructose is a rare monosaccharide that exists in extremely small quantities in nature, and it is also hard to prepare at a large scale via chemical or enzyme synthetic route due to low conversion and downstream separation complexity[1]. Importantly, a d-fructose decoration of 16% renders the polymers water-soluble and eliminates the cytotoxicity of PEI in noncancer L929 cells, accompanied by a reduced unspecific cellular uptake of the genetic material. The introduction of d-fructose shows superior potential for cell targeting, which can be assumed to be GLUT5 dependent[2]. Tracer D-[14C]-fructose uptake rate was reduced to 54.8% in 50 mM d-allulose and to 16.4% in 50 mM d-fructose, suggesting d-allulose competed with D-[14C]-fructose and the affinity of d-allulose for GLUT5 was lower than that of d-fructose. GLUT5 clearly mediates, likely at lower affinity relative to d-fructose, intestinal d-allulose transport[3]. The adsorbed-FDH electrodes catalyzed the electrochemical two-electron oxidation of d-fructose to 5-keto-d-fructose without a mediator. Nanostructured carbon particle-modified electrodes were used for the coulometric d-fructose biosensor to enhance the catalytic current density. The electric charge for the d-fructose oxidation gained by the biocoulometric measurement was in good agreement with the theoretical value corresponding to d-fructose amount in the range from 1 to 100 mM with a sample volume of 1 muL[4].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

5.55mL

1.11mL

0.56mL

27.75mL

5.55mL

2.78mL

55.51mL

11.10mL

5.55mL

参考文献

[1]Xiao Q, Niu J, Liu H, Liu Y, Zhou X. High Conversion of D-Fructose into D-Allulose by Enzymes Coupling with an ATP Regeneration System. Mol Biotechnol. 2019;61(6):432‐441

[2]Englert C, Pröhl M, Czaplewska JA, et al. d-Fructose-Decorated Poly(ethylene imine) for Human Breast Cancer Cell Targeting. Macromol Biosci. 2017;17(8):10.1002/mabi.201600502

[3]Kishida K, Martinez G, Iida T, Yamada T, Ferraris RP, Toyoda Y. d-Allulose is a substrate of glucose transporter type 5 (GLUT5) in the small intestine. Food Chem. 2019;277:604‐608

[4]Tsujimura S, Nishina A, Kamitaka Y, Kano K. Coulometric D-fructose biosensor based on direct electron transfer using D-fructose dehydrogenase. Anal Chem. 2009;81(22):9383‐9387