HEPES sodium

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Chemical Structure| 75277-39-3 同义名 : -
CAS号 : 75277-39-3
货号 : A260290
分子式 : C8H17N2NaO4S
纯度 : 98%
分子量 : 260.286
MDL号 : MFCD00036463
存储条件:

Pure form Inert atmosphere,Room Temperature

In solvent -20°C:3-6个月-80°C:12个月

溶解度 :

DMSO: 25 mg/mL(96.05 mM),配合低频超声助溶,注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO

动物实验配方:
生物活性
描述 HEPES sodium is a nonvolatile zwitterionic chemical buffer widely used in cell culture. HEPES sodium is effective at pH 6.8 to 8.2. HEPES sodium is also a potent inducer of lysosomal biogenesis[1][2][3].HEPES sodium maintains titanium superhydrophilicity for at least 3 months, resulting in sustained bioactivity and osteoconductivity[1].HEPES sodium drives lysosomal biogenesis, affects the cytoplasmic-nuclear distribution of MiT/TFE, and disrupts the overall transcriptional profile of the cell, thereby activating the MiT/TFE-dependent lysosomal-autophagy gene network in cultured RAW264.7 cells[3].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.84mL

0.77mL

0.38mL

19.21mL

3.84mL

1.92mL

38.42mL

7.68mL

3.84mL

参考文献

[1]Suzuki T, et al. Nonvolatile buffer coating of titanium to prevent its biological aging and for drug delivery. Biomaterials. 2010;31(18):4818-4828.

[2]Sledź P, et al. An experimental charge density of HEPES. Acta Crystallogr B. 2010;66(Pt 4):482-492.

[3]https://pubmed.ncbi.nlm.nih.gov/20631430/