Inosine

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Chemical Structure| 58-63-9 同义名 : 肌苷 ;9-β-D-Ribofuranosylhypoxanthine;NSC 20262;INO 495
CAS号 : 58-63-9
货号 : A257563
分子式 : C10H12N4O5
纯度 : 98%
分子量 : 268.226
MDL号 : MFCD00066770
存储条件：

Pure form Keep in dark place,Sealed in dry,Room Temperature

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(391.46 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 10 mg/mL(37.28 mM)，配合低频超声助溶
动物实验配方：
生物活性
描述 Inosine, a naturally occurring purine nucleoside, has been shown to stimulate axonal growth in cell culture and promote corticospinal tract axons to sprout collateral branches after stroke, spinal cord injury and TBI in rodent models[1]. Inosine-mediated A2AR activation leads to cAMP production with an EC50 of 300.7 μM and to extracellular signal-regulated kinase-1 and -2 (ERK1/2) phosphorylation with an EC50 of 89.38 μM. Inosine dose-dependently stimulates cAMP production mediated through the A2AR. Inosine dose-dependently induces A2AR-mediated ERK1/2 phosphorylation[2]. The administration of inosine exerted neuroprotective effects against EAE (experimental autoimmune encephalomyelitis) by diminishing clinical signs, including thermal and mechanical hyperalgesia, as well as weight loss typical of the disease. Up-regulation of IL-17 levels in the secondary lymphoid tissues, a result of EAE, was prevented by inosine treatment in EAE mice. Additionally, inosine consistently prevented A2AR up-regulation in the spinal cord, likely, through an ERK1-independent pathway. Graphical Abstract Preventive treatment with inosine inhibits the development and progression of EAE in C57Bl/6 mice. Furthermore, neuroinflammation and demyelinating processes were blocked by inosine treatment[3].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.73mL

0.75mL

0.37mL

18.64mL

3.73mL

1.86mL

37.28mL

7.46mL

3.73mL
参考文献

[1]Moore TL, Pessina MA, Finklestein SP, et al. Inosine enhances recovery of grasp following cortical injury to the primary motor cortex of the rhesus monkey. Restor Neurol Neurosci. 2016;34(5):827-848

[2]Welihinda AA, Kaur M, Greene K, Zhai Y, Amento EP. The adenosine metabolite inosine is a functional agonist of the adenosine A2A receptor with a unique signaling bias. Cell Signal. 2016;28(6):552-560

[3]Junqueira SC, Dos Santos Coelho I, Lieberknecht V, et al. Inosine, an Endogenous Purine Nucleoside, Suppresses Immune Responses and Protects Mice from Experimental Autoimmune Encephalomyelitis: a Role for A2A Adenosine Receptor. Mol Neurobiol. 2017;54(5):3271-3285