产品说明书
BCH
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同义名 : | 2-Amino-2-norbornanecarboxylic acid;LAT1-IN-1 |
| CAS号 : | 20448-79-7 | |
| 货号 : | A255246 | |
| 分子式 : | C8H13NO2 | |
| 纯度 : | 95% | |
| 分子量 : | 155.194 | |
| MDL号 : | MFCD00167580 | |
| 存储条件： |
粉末 Keep in dark place,Sealed in dry,Room Temperature 液体 -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
H2O: 20 mg/mL(128.87 mM)，配合低频超声，并水浴加热至45℃助溶 |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | L-Type amino acid transporter 1 (LAT1) is overexpressed in malignant tumor cells to support their growth and proliferation. BCH is a LAT1 inhibitor that inhibits the uptake of [14C]L-leucine (1μM) in KB, Saos2, and C6 cells with IC50 values of 75.3±6.7μM, 78.8±3.5μM, and 73.1±4.5μM, respectively. BCH also exhibited anti-proliferative effects on KB, Saos2, and C6 cells with IC50 values of 6.9±0.4mM, 5.6±0.7mM, and 6.5 ± 0.9mM, respectively. Treatment of cancer cells with 20 mM BCH significantly increased the proportion of TUNEL-positive cells in comparison to the untreated groups. BCH treatment also significantly promoted proteolytic cleavage of procaspase-7 and procaspase-3 in cancer cells.[1] Treatment of primary mouse hepatocytes with 10mM BCH led to 38% and 50% increase in cellular and extracellular levels of glutamate, respectively. In normal C57BL/6J mice, treatment with 0.7g/kg BCH for 6 hr significantly increased glutamate level, glutamate dehydrogenase activity, and NAD+/NADH ratio in the liver. In mice fed with high fat/high fructose western diet, peritoneal injection of BCH (0.7g/kg/day) for 8 weeks reduced the level of triglyceride by 30% compared to the control group. BCH treatment also reduced high fat/high fructose diet-induced stress, inflammation and ameliorated hepatic injury and hyperglycemia in mice.[2] | ||
| 作用机制 | BCH is a selective LAT1 inhibitor that can suppress cell growth and induce apoptosis.[1] | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
6.44mL 1.29mL 0.64mL |
32.22mL 6.44mL 3.22mL |
64.44mL 12.89mL 6.44mL |
| 参考文献 |
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