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ZLN024 HCl

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Chemical Structure| 1883548-91-1 同义名 : ZLN024 hydrochloride
CAS号 : 1883548-91-1
货号 : A247688
分子式 : C13H14BrClN2OS
纯度 : 99%+
分子量 : 361.685
MDL号 : MFCD28166492
存储条件：

粉末 Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 45 mg/mL(124.42 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 ZLN024 effectively stimulates both active AMPK heterotrimers and inactive α1 subunit truncations α1 (1-394) and α1 (1-335), although it does not affect α1 (1-312). For ZLN024 to activate AMPK, Thr-172 must be pre-phosphorylated by an upstream kinase, and ZLN024 also shields AMPK Thr-172 from dephosphorylation by PP2Cα. In L6 myotubes, ZLN024 activation of AMPK leads to increased glucose uptake and fatty acid oxidation without altering the ADP/ATP ratio. Identified through random screening using a scintillation proximity assay (SPA) targeting the AMPK α1β1γ1 heterotrimer, ZLN024 has been pinpointed as a novel AMPK activator. It activates AMPK α1β1γ1 and its homologue α2β1γ1 in a dose-dependent manner, enhancing the activity of α1β1γ1 by 1.5-fold with an EC50 of 0.42 µM, and that of α2β1γ1 by 1.7-fold with an EC50 of 0.95 µM. Additionally, ZLN024 boosts the activity of recombinant AMPK α1β2γ1 and α2β2γ1 by 1.7-fold and 1.6-fold, with EC50 values of 1.1 µM and 0.13 µM, respectively[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.76mL

0.55mL

0.28mL

13.82mL

2.76mL

1.38mL

27.65mL

5.53mL

2.76mL
参考文献

[1]Zhang LN, et al. Novel small-molecule AMP-activated protein kinase allosteric activator with beneficial effects in db/db mice. PLoS One. 2013 Aug 20;8(8):e72092.