产品说明书
CK-636
 |
同义名 : | CK-0944636 |
| CAS号 : | 442632-72-6 | |
| 货号 : | A237397 | |
| 分子式 : | C16H16N2OS | |
| 纯度 : | 99%+ | |
| 分子量 : | 284.376 | |
| MDL号 : | MFCD03036245 | |
| 存储条件： |
粉末 Keep in dark place,Sealed in dry,2-8°C 液体 -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 50 mg/mL(175.82 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | The Arp2/3 complex consists of seven evolutionarily conserved subunits (Arp2, Arp3 and ARPC1-5) and plays an essential role in polymerization of actin filaments which supports many types of cellular movements[3]. CK-636 is a inhibitor of Arp2/3 complex which could inhibit actin polymerization stimulated by Arp2/3 complex with IC50 values of 4 μM, 24 μM and 32 μM for human, fission yeast and bovine, respectively[3]. By the THP-1 monocyte cell line, CK-636 inhibited the formation of podosomes which are adhesive structures depending on Arp2/3 complex, WASP (Wiskott–Aldrich syndrome protein) and actin polymerization[3]. When the formation of lamellipodia was inhibited by CK-636, a substantial fraction of T cells on those surfaces containing zigzag patterns with an acute turning angle were trapped at the interfaces formed by the turning points of the zigzag patterns[4]. | ||
| 作用机制 | CK-636 binds in a pocket between subdomain 4 of Arp2 and subdomain 1 of Arp3. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
3.52mL 0.70mL 0.35mL |
17.58mL 3.52mL 1.76mL |
35.16mL 7.03mL 3.52mL |
| 参考文献 |
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