产品说明书

Pirodavir

Print
Chemical Structure| 124436-59-5 同义名 : R77975
CAS号 : 124436-59-5
货号 : A232986
分子式 : C21H27N3O3
纯度 : 99%+
分子量 : 369.457
MDL号 : MFCD00866965
存储条件：

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 9 mg/mL(24.36 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Pirodavir demonstrates potent broad-spectrum activity against picornaviruses. It effectively inhibits 80 out of 100 tested human rhinovirus (HRV) strains at a concentration of 64 ng/mL. Additionally, Pirodavir shows effectiveness against 16 enteroviruses, with a mean IC80 of 1,300 ng/mL. Moreover, it inhibits enterovirus 71 replication with an IC50 of 5,420 nM and an IC90 exceeding 13,350 nM. Pirodavir also inhibits 56 laboratory strains of rhinovirus and three clinical isolates, encompassing 59% of the serotypes and isolates with IC50s of less than 100 nM [1]. Pirodavir concentrations of 16 and 4 μg/mL result in a reduction of cell growth by 66% (standard error of the mean [s.e.M.] 0.75) and 28% (s.e.M. 0.25), respectively. Lower concentrations (1 μg/mL) of Pirodavir do not exhibit inhibitory effects on cell growth. The 50% cytotoxic concentration of pirodavir for logarithmic cell growth at 37°C is determined to be 7 μg/mL. However, under the conditions of the antiviral assay (confluent HeLa cells at 33°C), the 50% cytotoxic concentration exceeds 50 μg/mL[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.71mL

0.54mL

0.27mL

13.53mL

2.71mL

1.35mL

27.07mL

5.41mL

2.71mL
参考文献

[1]Barnard DL, et al. In vitro activity of expanded-spectrum pyridazinyl oxime ethers related to pirodavir: novel capsid-binding inhibitors with potent antipicornavirus activity. Antimicrob Agents Chemother. 2004 May;48(5):1766-72.

[2]Andries K, et al. In vitro activity of pirodavir (R 77975), a substituted phenoxy-pyridazinamine with broad-spectrum antipicornaviralactivity. Antimicrob Agents Chemother. 1992 Jan;36(1):100-7.