产品说明书
Floxuridine
 |
同义名 : | 氟尿苷 ;5-Fluorouracil 2'-deoxyriboside;5-FDU;FUdR. WR138720.;FDUR;fluoruridine deoxyribose. FUDF. 5FUDR;fluorouridine deoxyribose;fluorodeoxyuridine;floxuridin;NSC 26740;5-FUDR;5-Fluorodeoxyuridine;FdUrd;NSC 27640 |
| CAS号 : | 50-91-9 | |
| 货号 : | A230277 | |
| 分子式 : | C9H11FN2O5 | |
| 纯度 : | 98% | |
| 分子量 : | 246.192 | |
| MDL号 : | MFCD00006530 | |
| 存储条件： |
粉末 Inert atmosphere,2-8°C 液体 -20°C:3-6个月-80°C:12个月 |
|
| 溶解度 : |
DMSO: 120 mg/mL(487.42 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO H2O: 50 mg/mL(203.09 mM) |
|
| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | Floxuridine (5-Fluorouracil 2'-deoxyriboside) is a pyrimidine analog and known as an oncology antimetabolite. Floxuridine (0-25 μM; 4-24 hours) is affectd by inhibitors of PARP and its sensitivity of ovarian cancer cells is enhanced. Co-exposed to FdUrd and the PARP inhibitor markedly increases killing cell numbers when its compare to treatment alone in ovarian cancer cells. Floxuridine (300 μM; 4-24 hours) increases p-Chk1 and p-Chk2 in ovarian cancer cell lines. It may induce DNA damage and activate the ATM and ATR checkpoint signaling pathways. Floxuridine (0-2.5 μM; 24 hours) causes a G1/S-phase arrest and following removal of the FdUrd, the G1/S-phase-arrested cells moved synchronously through S phase and into G2/M[3]. Floxuridine (FUDR) is an ideal drug for hepatic arterial infusion (HAI) due to its short half life, steep dose response curve, high total body clearance, and high hepatic extraction[4]. Floxuridine is against Mueller Hinton Broth and Tryptic Soy Broth with MIC values of 0.25 μM and 0.81 μM, respectively. It also reported to be a very potent inhibitor of staphylococcal growth (MIC, 0.025–0.00313 μM)[5]. | ||
| 细胞研究 | |||||
|---|---|---|---|---|---|
| 细胞系 | 浓度 | 检测类型 | 检测时间 | 活动说明 | 数据源 |
| 786-0 cells | Function assay | 48 h | Anticancer activity against human 786-0 cells after 48 hrs by sulforhodamine B assay, GI50=0.1 μM | 19744858 | |
| A2780 cells | Cytotoxicity assay | 5 days | Cytotoxicity against human A2780 cells after 5 days by MTT assay, IC50=0.026 μM | 22738636 | |
| A498 cells | Function assay | 48 h | Anticancer activity against human A498 cells after 48 hrs by sulforhodamine B assay, GI50=1.25893 μM | 19744858 | |
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
|
1 mM 5 mM 10 mM |
4.06mL 0.81mL 0.41mL |
20.31mL 4.06mL 2.03mL |
40.62mL 8.12mL 4.06mL |
| 参考文献 |
|---|