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BAPTA-AM

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Chemical Structure| 126150-97-8 同义名 : BAPTA Acetoxymethyl ester
CAS号 : 126150-97-8
货号 : A229213
分子式 : C34H40N2O18
纯度 : 95%
分子量 : 764.684
MDL号 : MFCD00036696
存储条件：

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(65.39 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 BAPTA-AM is a well-known membrane permeable Ca(2+) chelator. The externally applied BAPTA-AM inhibited hERG channels in a concentration-dependent manner (IC(50): 1.3 microM). BAPTA-AM inhibited hKv1.3 and hKv1.5 channels in a concentration-dependent manner (IC(50): 1.45 and 1.23 microM, respectively), and the blockade of these two types of channels was also dependent on channel opening[3]. BAPTA-AM (BAPTA/AM), an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures. Mixed cortical cell cultures (DIV 13-16) exposed to 10 μM BAPTA-AM for 24- or 48-hr show moderate (45-70%) neuronal injury as evaluated by increased LDH release into the bathing medium after 24-48-hr. Exposure of cortical cultures to 3-10 μM BAPTA-AM for 48-hr evoke dose-dependent neuronal damage[4]. BAPTA-AM inhibit osteoclastogenic ability of BMMs via suppressing the increase of [Ca(2+)](i) which lead to inhibit RANKL-induced the phosphorylation of ERK, Akt and p38 MAPK, but not JNK[5]. BAPTA-AM can decrease cellular pH and inhibit acidocalcisome acidification in starved cells[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.31mL

0.26mL

0.13mL

6.54mL

1.31mL

0.65mL

13.08mL

2.62mL

1.31mL
参考文献

[1]Tang Q, Jin MW, et al. The membrane permeable calcium chelator BAPTA-AM directly blocks human ether a-go-go-related gene potassium channels stably expressed in HEK 293 cells. Biochem Pharmacol. 2007 Dec 3;74(11):1596-607.

[2]Wie MB, Koh JY, et al. BAPTA/AM, an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures. Prog Neuropsychopharmacol Biol Psychiatry. 2001 Nov;25(8):1641-59.

[3]Tang Q, Jin MW, Xiang JZ, Dong MQ, Sun HY, Lau CP, Li GR. The membrane permeable calcium chelator BAPTA-AM directly blocks human ether a-go-go-related gene potassium channels stably expressed in HEK 293 cells. Biochem Pharmacol. 2007 Dec 3;74(11):1596-607

[4]Wie MB, Koh JY, Won MH, Lee JC, Shin TK, Moon CJ, Ha HJ, Park SM, Kim HC. BAPTA/AM, an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures. Prog Neuropsychopharmacol Biol Psychiatry. 2001 Nov;25(8):1641-59

[5]Zhou S, Yuan X, Liu Q, Zhang X, Pan X, Zang L, Xu L. BAPTA-AM, an intracellular calcium chelator, inhibits RANKL-induced bone marrow macrophages differentiation through MEK/ERK, p38 MAPK and Akt, but not JNK pathways. Cytokine. 2010 Dec;52(3):210-4

[6]Li FJ, Tan KS, He CY. BAPTA-AM decreases cellular pH, inhibits acidocalcisome acidification and autophagy in amino acid-starved T. brucei. Mol Biochem Parasitol. 2017 Apr;213:26-29