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Ferrostatin-1

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Chemical Structure| 347174-05-4 同义名 : Fer-1
CAS号 : 347174-05-4
货号 : A228648
分子式 : C15H22N2O2
纯度 : 98%
分子量 : 262.34
MDL号 : MFCD08072959
存储条件：

粉末 Keep in dark place,Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 120 mg/mL(457.42 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：

2% DMSO+50% PEG 300+5% Tween 80+water 5 mg/mL

生物活性
靶点

  • Ferroptosis

    Ferroptosis, EC50:60 nM
描述 Ferrostatin-1 (Fer-1), known for its potent inhibition of ferroptosis, effectively blocks the ferroptosis triggered by Erastin in HT-1080 cells, with an EC50 value of 60 nM. As a synthetic antioxidant, Ferrostatin-1 operates through a reductive action to shield membrane lipids from damage, thus preventing cellular death. Additionally, it demonstrates antifungal properties[1][2][3]. Ferrostatin-1 thwarts the build-up of cytosolic and lipid reactive oxygen species (ROS) caused by erastin. It also protects against glutamate-triggered neurotoxicity in organotypic slices of rat brain[1]. At a concentration of 2 μM for 24 hours, Ferrostatin-1 safeguards against neurotoxicity induced by 5 mM Glutamate in rat organotypic hippocampal slice cultures (OHSC). While Ferrostatin-1 impedes lipid peroxidation, it does not affect the formation of mitochondrial ROS or the permeability of lysosomal membranes. It also prevents cell death in models of Huntington's disease (HD), periventricular leukomalacia (PVL), and kidney dysfunction[2]. At a dosage of 1 μM over 6 hours, Ferrostatin-1 hinders the oxidative degradation of unsaturated fatty acids in HT-1080 cells, leading to an increase in the count of healthy medium spiny neurons (MSNs)[3].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.81mL

0.76mL

0.38mL

19.06mL

3.81mL

1.91mL

38.12mL

7.62mL

3.81mL
参考文献

[1]Dixon SJ, et al. Ferroptosis: an iron-dependent form of nonapoptotic cell death. Cell. 2012;149(5):1060-1072.

[2]Skouta R, Dixon SJ, Wang J, et al. Ferrostatins inhibit oxidative lipid damage and cell death in diverse disease models. J Am Chem Soc. 2014;136(12):4551-4556.

[3]Horwath MC, et al. Antifungal Activity of the Lipophilic Antioxidant Ferrostatin-1. Chembiochem. 2017;18(20):2069-2078.

[4]Liu P, Feng Y, et al. Ferrostatin-1 alleviates lipopolysaccharide-induced acute lung injury via inhibiting ferroptosis. Cell Mol Biol Lett. 2020;25:10. Published 2020 Feb 27.

[5]Melania Guerrero Hue, et al. FP282 FERROPTOSIS-MEDIATED CELL DEATH IS DECREASED BY CURCUMIN IN RENAL DAMAGE ASSOCIATED TO RHABDOMYOLYSIS, Nephrology Dialysis Transplantation, Volume 34, Issue Supplement_1, June 2019, gfz106.FP282.

[6]Zhang M, et al. Ferrostatin-1 attenuates hypoxic-ischemic brain damage in neonatal rats by inhibiting ferroptosis. Transl Pediatr. 2023 Nov 28;12(11):1944-1970.

[7]Cheon YI, et al. Effect of deferoxamine and ferrostatin-1 on salivary gland dysfunction in ovariectomized rats. Aging (Albany NY). 2023 Apr 6;15(7):2418-2432.