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Kaempferitrin

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Chemical Structure| 482-38-2 同义名 : Lespenephryl;Lespedin;Kaempferol 3,7-bisrhamnoside;4-18-00-03292 (Beilstein Handbook Reference);Kaempferol 3,7-O-α-Dirhamnoside;Grosvenorine II;BRN 0073958
CAS号 : 482-38-2
货号 : A224689
分子式 : C27H30O14
纯度 : 95%
分子量 : 578.519
MDL号 : MFCD01662671
存储条件：

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 25 mg/mL(43.21 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Kaempferitrin (KM) is a natural flavonoid, possesses antinociceptive, anti-inflammatory, anti-diabetic, antitumoral and chemopreventive effects, and activates insulin signaling pathway. Kaempferitrin causes survival rates higher than 90% at 1-20 μM in matured 3T3-L1 adipocyte, and the survival rates decline rapidly at 25 and 50 μM. Kaempferitrin (15 μM) increases insulin receptor beta tyrosine phosphorylation and tyrosine phosphorylation of the insulin receptor substrate 1, and such effects are similar to that of 10 nM insulin. In addition, Kaempferitrin increases the total levels of Glu4 protein in differentiated cells and secreted adiponectin in mature 3T3-L1 adipocytes[3]. KM induces high cytotoxic effects in vitro and in vivo against HeLa cells. Kaempferitrin (2.5, 10 and 25 mg/kg, i.p.) markedly suppresses the growth of tumor by 40%, 87% and 97%, and decreases tumor weight by 37%, 81% and 95%, respectively in nu/nu mice bearing HeLa tumor[4]. Kaempferitrin decreased the levels of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, matrix metalloproteinase (MMP)-1, and MMP-3 in MH7A cells. Moreover, kaempferitrin blocked the activation of nuclear factor-κB (NF-κB) and protein kinase B (Akt)/mammalian target of rapamycin (mTOR) pathways. Furthermore, treatment with kaempferitrin decreased paw thickness and arthritis scores, and reduced the serum levels of IL-1β, IL-6, and TNF-α in a collagen-induced arthritis mouse model[5]. KM effectively increased SOD (Superoxide dismutase) activity, decreased MDA (malondialdehyde) levels, suppressed ROS (Reactive oxygen species) generation, and protected against OS (oxidative stress) in AGE-induced (Advanced glycation end products) GMCs[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.73mL

0.35mL

0.17mL

8.64mL

1.73mL

0.86mL

17.29mL

3.46mL

1.73mL
参考文献

[1]Da Silva D, Casanova LM, et al. Antidiabetic activity of Sedum dendroideum: metabolic enzymes as putative targets for the bioactive flavonoid kaempferitrin. IUBMB Life. 2014 May;66(5):361-70.

[2]Alonso-Castro AJ, Ortiz-Sanchez E, et al. Kaempferitrin induces apoptosis via intrinsic pathway in HeLa cells and exerts antitumor effects. J Ethnopharmacol. 2013 Jan 30;145(2):476-89.

[3]Tzeng YM, Chen K, Rao YK, Lee MJ. Kaempferitrin activates the insulin signaling pathway and stimulates secretion of adiponectin in 3T3-L1 adipocytes. Eur J Pharmacol. 2009;607(1-3):27-34.

[4]Alonso-Castro AJ, Ortiz-Sánchez E, García-Regalado A, et al. Kaempferitrin induces apoptosis via intrinsic pathway in HeLa cells and exerts antitumor effects. J Ethnopharmacol. 2013;145(2):476-489

[5]Wang J, Zhao Q. Kaempferitrin inhibits proliferation, induces apoptosis, and ameliorates inflammation in human rheumatoid arthritis fibroblast-like synoviocytes. Phytother Res. 2019;33(6):1726-1735

[6]Jiang W, Wang R, Liu D, et al. Protective Effects of Kaempferitrin on Advanced Glycation End Products Induce Mesangial Cell Apoptosis and Oxidative Stress. Int J Mol Sci. 2018;19(11):3334. Published 2018 Oct 26