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NMS-P715

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Chemical Structure| 1202055-32-0 同义名 : -
CAS号 : 1202055-32-0
货号 : A223438
分子式 : C35H39F3N8O3
纯度 : 98+%
分子量 : 676.731
MDL号 : MFCD19443684
存储条件：

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 2 mg/mL(2.96 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 NMS-P715 is a selective MPS1 inhibitor with an IC50 of 182 nM. It is highly specific, affecting no kinases below a 5 μM IC50 and only inhibiting CK2, MELK, and NEK6 under 10 μM. NMS-P715 triggers a significant spindle assembly checkpoint (SAC) bypass with an EC50 of 65 nM. At 1 μM, it accelerates mitosis in U2OS cells expressing YFP-α-tubulin, leads to aneuploidy, and suppresses HCT116 cell proliferation. Additionally, NMS-P715 (0.5, 1 μM) impacts the stability of the mitotic checkpoint complex (MCC) and cdc20 ubiquitination[1]. NMS-P715 (1 μM) enables spindle assembly checkpoint bypass and induces apoptosis in pancreatic ductal adenocarcinoma (PDAC) cell lines. Additionally, NMS-P715 (0-25 μM) selectively curtails the growth of PDAC cells[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.48mL

0.30mL

0.15mL

7.39mL

1.48mL

0.74mL

14.78mL

2.96mL

1.48mL
参考文献

[1]Colombo R, et al. Targeting the mitotic checkpoint for cancer therapy with NMS-P715, an inhibitor of MPS1 kinase. Cancer Res. 2010 Dec 15;70(24):10255-64.

[2]Slee RB, et al. Selective inhibition of pancreatic ductal adenocarcinoma cell growth by the mitotic MPS1 kinase inhibitor NMS-P715. Mol Cancer Ther. 2014 Feb;13(2):307-315.