产品说明书

W-7 hydrochloride

Print
Chemical Structure| 61714-27-0 同义名 : -
CAS号 : 61714-27-0
货号 : A201910
分子式 : C16H22Cl2N2O2S
纯度 : 98%
分子量 : 377.33
MDL号 : MFCD00012559
存储条件：

粉末 Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 250 mg/mL(662.55 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 1 mg/mL(2.65 mM)，配合低频超声，并水浴加热至45℃助溶
动物实验配方：
生物活性
描述 W-7 hydrochloride selectively antagonizes calmodulin. It hinders both the Ca2+-calmodulin-dependent phosphodiesterase and the myosin light chain kinase, exhibiting IC50 values of 28 μM for the former and 51 μM for the latter[1][2]. W-7 hydrochloride is known to induce apoptosis and possesses antitumor properties[3]. Predominantly located in the cytoplasm, W-7 hydrochloride suppresses the proliferation of CHO-K1 cells. It specifically impedes the cell cycle at the G1/S boundary phase, with 25 μM of W-7 hydrochloride halting cell growth at this stage[1]. At a concentration of 100 μM, W-7 displays comparable antagonistic effects on the contractile reactions to both carbachol and KCl. W-7 also inhibits the rise in phosphorylated myosin light chain (P-LC) levels that is typically observed following a 1-minute exposure to 10 μM carbachol. This inhibition by W-7 ultimately counteracts smooth muscle contraction by preventing the initial surge in P-LC phosphorylation[2]. Administering W-7 leads to a dose-responsive curtailment of cell growth across various human multiple myeloma cell lines. It prompts G1 phase arrest in the cell cycle by diminishing cyclins and elevating p21cip1 levels. Furthermore, W-7 triggers apoptosis through the activation of caspases, a process that is partially attributed to the increase in intracellular calcium and the depolarization of the mitochondrial membrane potential, as well as the inhibition of STAT3 phosphorylation, which results in the reduced expression of the Mcl-1 protein[3].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.65mL

0.53mL

0.27mL

13.25mL

2.65mL

1.33mL

26.50mL

5.30mL

2.65mL
参考文献

[1]H Hidaka, et al. N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide, a Calmodulin Antagonist, Inhibits Cell Proliferation. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4354-7.

[2]M Asano. Divergent Pharmacological Effects of Three Calmodulin Antagonists, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), Chlorpromazine and Calmidazolium, on Isometric Tension Development and Myosin Light Chain Phosphorylation in Intact Bovin

[3]H Itoh, et al. Direct Interaction of Calmodulin Antagonists With Ca2+/calmodulin-dependent Cyclic Nucleotide Phosphodiesterase. J Biochem. 1984 Dec;96(6):1721-6.