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NADP disodium salt

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Chemical Structure| 24292-60-2 同义名 : beta-烟酰胺腺嘌呤二核苷酸磷酸二钠盐 ;Disodium NADP
CAS号 : 24292-60-2
货号 : A198764
分子式 : C21H26N7Na2O17P3
纯度 : 99%
分子量 : 787.369
MDL号 : MFCD00065390
存储条件:

粉末 Keep in dark place,Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月

溶解度 :

DMSO: 3 mg/mL(3.81 mM),配合低频超声,并水浴加热至45℃助溶,注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO

H2O: 250 mg/mL(317.51 mM),配合低频超声助溶

动物实验配方:
生物活性
描述 NADP disodium salt (Disodium NADP), a nicotinamide adenine dinucleotide, is a redox cofactor. NADP disodium salt is a key cofactor for electron transfer in the metabolism, being alternately oxidized (NADP+) and reduced (NADPH). NADP(+), the oxidized form of nicotinamide adenine dinucleotide phosphate, plays an essential role as a coenzyme in cellular electron transfer reactions. The concentration of NADP(+) in cytoplasm or organelles is dynamic due to its conversion to many important derivatives[1]. NADP differs from NAD in only the presence of an additional phosphate group esterified to the 2'-hydroxyl group of the ribose at the adenine end and yet NADP is confined with few exceptions to the reactions of reductive biosynthesis, whereas NAD is used almost exclusively in oxidative degradations[2]. NADP is the preferred coenzyme for the oxidative deamination of glutamate by GDH (glutamate dehydrogenase) even though the enzyme is capable of utilizing either coenzyme in vitro[3].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.27mL

0.25mL

0.13mL

6.35mL

1.27mL

0.64mL

12.70mL

2.54mL

1.27mL

参考文献

[1]Zhao FL, Zhang C, Zhang C, Tang Y, Ye BC. A genetically encoded biosensor for in vitro and in vivo detection of NADP(.). Biosens Bioelectron. 2016 Mar 15;77:901-6.

[2]Carugo O, Argos P. NADP-dependent enzymes. I: Conserved stereochemistry of cofactor binding. Proteins. 1997 May;28(1):10-28.

[3]Male KB, Storey KB. Regulation of coenzyme utilization by bovine liver glutamate dehydrogenase: investigations using thionicotinamide analogues of NAD and NADP in a dual wavelength assay. Int J Biochem. 1982;14(12):1083-9.