产品说明书

Dihydrofluorescein diacetate

Print
Chemical Structure| 35340-49-9 同义名 : -
CAS号 : 35340-49-9
货号 : A192744
分子式 : C24H18O7
纯度 : 98%
分子量 : 418.396
MDL号 : MFCD00005056
存储条件：

粉末 Sealed in dry,Room Temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 100 mg/mL(239.01 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Dihydrofluorescein diacetate may be an excellent fluorescent probe for many cell-based studies. It is a better fluorescent probe for detecting intracellular oxidants because it is more reactive to specific oxidants.Dihydrofluorescein diacetate exhibits fluorescence in reoxygenated endothelial cells with a linear structure consistent with mitochondria[1].Dihydrofluorescein diacetate detects the presence of ROS in mitochondria.Dihydrofluorescein diacetate fluoresces clearly, depicting thin filaments, all of which correspond in detail to TMRM-stained mitochondria. It enters the mitochondria and reacts with ROS released in the matrix[2].Dihydrofluorescein diacetate may be a useful quantitative method for measuring the oxidation potential of nanoparticle-treated cells [3].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.39mL

0.48mL

0.24mL

11.95mL

2.39mL

1.20mL

23.90mL

4.78mL

2.39mL
参考文献

[1]Hempel SL, et al. Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free Radic Biol Med. 1999 Jul;27(1-2):146-59.

[2]Diaz G, et al. Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes. Histochem Cell Biol. 2003 Oct;120(4):319-25.

[3]Aranda A, et al. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay: a quantitative method for oxidative stress assessment of nanoparticle-treated cells. Toxicol In Vitro. 2013 Mar;27(2):954-63.