产品说明书
NMDA
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同义名 : | (R)-2-(甲氨基)丁二酸 ;N-Methyl-D-aspartic acid |
| CAS号 : | 6384-92-5 | |
| 货号 : | A188283 | |
| 分子式 : | C5H9NO4 | |
| 纯度 : | 98% | |
| 分子量 : | 147.129 | |
| MDL号 : | - | |
| 存储条件： |
粉末 Sealed in dry,Room Temperature 液体 -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 9 mg/mL(61.17 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO H2O: 30 mg/mL(203.9 mM)，配合低频超声助溶 |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | NMDA is a specific agonist for NMDA receptor mimicking the action of glutamate, the neurotransmitter which normally acts at that receptor. Addition (0.1-10 mM) of N-methyl-D-aspartic acid (NMDA), one of the agonists for central excitatory amino acid neurotransmitter receptors, exerted a significant augmentation of the adrenal binding independently of the incubation temperature in a concentration-dependent manner[3]. Intracellular glass electrode recordings of single cell in current clamp and two-electrode voltage clamp modes indicate that glutamate (Glu, 0.1-1.0 mM) and NMDA (0.01-1.0 mM) increase electrically induced AP amplitude by hyperpolarising excitation threshold potential (Eth) and prolong AP (action potentials) fast repolarisation phase[4]. NMDA preconditioning reduced the maximal severity 6 displayed in QA(quinolinic acid)-administered mice, inducing protection in 47.6% of mice after QA-induced seizures[5]. Activation of NMDA-receptors is a necessary step of induction of LTP(long-term potentiation), whereas maintenance of LTP related mainly with increase of AMPA(alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate) -EPSP (excitatory postsynaptic potential)[6]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
6.80mL 1.36mL 0.68mL |
33.98mL 6.80mL 3.40mL |
67.97mL 13.59mL 6.80mL |
| 参考文献 |
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