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Nicotinamide

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Chemical Structure| 98-92-0 同义名 : 3-(氨基羰基)吡啶 ;Niacinamide;Nicotinic acid amide;Vitamin B3 Amide;NSC 27452;NSC 13128
CAS号 : 98-92-0
货号 : A187855
分子式 : C6H6N2O
纯度 : 99+%
分子量 : 122.125
MDL号 : MFCD00006395
存储条件：

粉末 Keep in dark place,Sealed in dry,Room Temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(409.42 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 50 mg/mL(409.42 mM)
动物实验配方：
生物活性
靶点

  • Sirtuin
描述 There are two types of enzymes that affect the acetylationstate of histones: histone acetyltransferases and the opposinghistone deacetylases (HDACs). Yeast silent information regulator 2 (Sir2) is the founding member of Class III HDACs. Nonredox enzyme, sirtuin 1 (Sirt1) is an important member of the mammalian sirtuin family of proteins that commonly have dual activities as a nicotinamide adenine dinucleotide (NAD+)- dependent deacetylase and as a mono-ADP ribosyltransferase. Nicotinamide (NAM), a form of vitamin B3, is an inhibitor of yeast Sir2 and human Sirt1 with IC50 < 50 μM. Treatment of the fetal cells with 10 mM NIC resulted in a twofold increase in DNA content and a threefold increase in insulin content and induced differentiation and maturation of human fetal pancreatic islet cells[3]. Nicotinamide incubation (5 mM) 2h prior to hypoxia significantly increased total nicotinamide adenine dinucleotide (NADH) content, improved neuronal recovery, enhanced ATP content, and prevented NADH hyperoxidation in hippocampal slices[4]. It was found that nicotinamide (200 mg/kg/day) treatment in Alzheimer’s disease transgenic mice-drinking water from birth until 4 months of age improved both the short- and long term memory[5].
作用机制 Nicotinamide inhibits deacetylation by binding to a conserved pocket adjacent to NAD+.
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
Escherichia coli BL21 (DE3) cells Function assay Inhibition of catalytically active human SIRT3 (102 to 399 amino acids) expressed in Escherichia coli BL21 (DE3) cells using fluorogenic 7-amino-4-methylcoumarin (AMC)-labeled peptide by fluorescence assay, IC50=6.2 μM 25275824
human SK-MEL-28 cells 100 μM Function assay 24 h Reduction in ATP level in human SK-MEL-28 cells at 100 uM maintained in Locke's solution after 24 hrs by luciferase-based assay in absence of GF and glucose 22835719
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

8.19mL

1.64mL

0.82mL

40.94mL

8.19mL

4.09mL

81.88mL

16.38mL

8.19mL
参考文献

[1]Bitterman KJ, Anderson RM, et al. Inhibition of silencing and accelerated aging by nicotinamide, a putative negative regulator of yeast sir2 and human SIRT1. J Biol Chem. 2002 Nov 22;277(47):45099-107.

[2]Otonkoski T, Beattie GM, et al. Nicotinamide is a potent inducer of endocrine differentiation in cultured human fetal pancreatic cells. J Clin Invest. 1993 Sep;92(3):1459-66

[3]Otonkoski T, Beattie GM, Mally MI, Ricordi C, Hayek A. Nicotinamide is a potent inducer of endocrine differentiation in cultured human fetal pancreatic cells. J Clin Invest. 1993 Sep;92(3):1459-66. doi: 10.1172/JCI116723. PMID: 8104197; PMCID: PMC288291.

[4]Shetty PK, Galeffi F, Turner DA. Nicotinamide pre-treatment ameliorates NAD(H) hyperoxidation and improves neuronal function after severe hypoxia. Neurobiol Dis. 2014 Feb;62:469-78. doi: 10.1016/j.nbd.2013.10.025. Epub 2013 Oct 31. PMID: 24184921; PMCID: PMC4143422.

[5]Green KN, Steffan JS, Martinez-Coria H, Sun X, Schreiber SS, Thompson LM, LaFerla FM. Nicotinamide restores cognition in Alzheimer's disease transgenic mice via a mechanism involving sirtuin inhibition and selective reduction of Thr231-phosphotau. J Neurosci. 2008 Nov 5;28(45):11500-10. doi: 10.1523/JNEUROSCI.3203-08.2008. PMID: 18987186; PMCID: PMC2617713.