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8-Azaadenosine

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Chemical Structure| 10299-44-2 同义名 : NSC 72961
CAS号 : 10299-44-2
货号 : A186525
分子式 : C9H12N6O4
纯度 : 97%
分子量 : 268.229
MDL号 : MFCD01631207
存储条件:

粉末 Keep in dark place,Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月

溶解度 :

DMSO: 250 mg/mL(932.04 mM),配合低频超声助溶,注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO

H2O: 3 mg/mL(11.18 mM),配合低频超声,并水浴加热至45℃助溶

动物实验配方:
生物活性
描述 Adenosine deaminases acting on RNA (ADARs) convert adenosine to inosine in double-stranded RNA. A-to-I editing of RNA is a widespread posttranscriptional process that has recently emerged as an important mechanism in cancer biology[2]. 8-Azaadenosine is a potent ADAR1 inhibitor and an A-to-I editing inhibitor. 8-Azaadenosine blocks RNA editing and inhibits proliferation, 3D growth, invasion, and migration in thyroid cancer cells. 8-Azaadenosine targets ADAR (adenosine deaminases acting on double-stranded RNA) activity only and does not affect ADAR1 mRNA levels. 8-Azaadenosine (10-25 nM) restores let-7 miRNA biogenesis commensurate with a reduction in ADAR1 expression, RNA editing activity and LIN28B expression in JAK2/BCR-ABL1 transduced progenitors after two weeks in stromal co-culture. 8-Azaadenosine (10, 100 nM) shows no effect on BCR-ABL and JAK2 signaling[1]. 8-Azaadenosine (0.1, 0.5, 1, 2 µM; 5 days) decreases cell viability/proliferation in a dose-dependent manner in TPC1 and Cal62 cells. 8-Azaadenosine (1, 2 µM) impedes invasion and migration and inhibits the editing activity of TPC1 and Cal62 cells. By contrast, ADAR1 mRNA levels remains stable in both cells lines[2]. In H. Ep. 2 cells, 8-aza-HR blocked the conversion of orotic acid to uridine nucleotides and caused an accumulation of orotidine. This inhibition of pyrimidine biosynthesis apparently does not contribute significantly to the cytotoxicity of 8-aza-HR because uridine provided no degree of reversal of its inhibition of the growth of cell cultures[3].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.73mL

0.75mL

0.37mL

18.64mL

3.73mL

1.86mL

37.28mL

7.46mL

3.73mL

参考文献

[1]Maria Anna Zipeto, et al. ADAR1 Activation Drives Leukemia Stem Cell Self-Renewal by Impairing Let-7 Biogenesis. Cell Stem Cell. 2016 Aug 4;19(2):177-191.

[2]Julia Ramírez-Moya, et al. ADAR1-mediated RNA editing is a novel oncogenic process in thyroid cancer and regulates miR-200 activity. Oncogene. 2020 Apr;39(18):3738-3753.

[3]1Bennett L L , Allan P W . Metabolism and Metabolic Effects of 8-Azainosine and 8-Azaadenosine. Cancer Research, 1976, 36(11 Pt 1):3917.