产品说明书
7-Aminocephalosporanic acid
 |
同义名 : | 7-ACA |
| CAS号 : | 957-68-6 | |
| 货号 : | A183629 | |
| 分子式 : | C10H12N2O5S | |
| 纯度 : | 98% | |
| 分子量 : | 272.28 | |
| MDL号 : | MFCD00005177 | |
| 存储条件： |
粉末 Keep in dark place,Sealed in dry,2-8°C 液体 -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
H2O: 1 mg/mL(3.67 mM)，配合低频超声，并调节pH至14 |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | 7-Aminocephalosporanic acid(7-ACA) is the core chemical structure for the synthesis of cephalosporin antibiotics, is a potent β-lactamase inhibitor[1]. The N-phenylacetyl derivative of 7-aminocephalosporanic acid (cephaloram) had roughly the same activity as benzylpenicillin against a number of Gram-positive organisms and about one-eighth of the activity of benzylpenicillin against penicillinsensitive strains of Staphylococcus aureus. This derivative and the N-alpha-phenoxypropionyl derivative of 7-aminocephalosporanic acid were 4 to 8 and 4 to 16 times as active as methicillin against penicillinase- and nonpenicillinase-producing staphylococcal strains, respectively. After forty-eight subcultures in the presence of antibiotic the increase in minimum inhibitory concentration against the staphylococcus was about four-times as great for cephaloram as for cephalosporin C. The resistant penicillinase-producing strains remained stable after six subcultures in antibiotic-free medium, and all the strains retained coagulase activity[2]. 7-ACA is produced from cephalosporin C via glutaryl-7-ACA (GL-7-ACA) by a bioconversion process using d-amino acid oxidase and cephalosporin acylase (or GL-7-ACA acylase). The best mutant enzyme for catalytic efficiency, with a k(cat)/K(m) value for GL-7-ACA almost 50-fold higher than that of the D433N enzyme, has three amino acid substitutions: D433N, Y444A, and G484A[3]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
|
1 mM 5 mM 10 mM |
3.67mL 0.73mL 0.37mL |
18.36mL 3.67mL 1.84mL |
36.73mL 7.35mL 3.67mL |
| 参考文献 |
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