Pluripotin

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Chemical Structure| 839707-37-8 同义名 : SC1
CAS号 : 839707-37-8
货号 : A183088
分子式 : C27H25F3N8O2
纯度 : 98+%
分子量 : 550.535
MDL号 : MFCD20527253
存储条件：

Pure form Keep in dark place,Sealed in dry,Store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 25 mg/mL(45.41 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
靶点

  • ERK1

    ERK1, Kd:98 nM
描述 Extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) are members of the mitogen-activated protein kinase super family that can mediate cell proliferation and apoptosis[3]. Pluripotin is a dual inhibitor of ERK1 and RasGAP with KDs of 98 nM and 212 nM, respectively. Pluripotin also inhibits RSK1, RSK2, RSK3, and RSK4 with IC50s of 0.5, 2.5, 3.3, and 10.0 µM, respectively[4]. Pluripotin (SC-1) inhibits Abl1, p70S6K, PLK2, RSK1, RSK2, RSK3, RSK4 with IC50s of 0.005, 1.4, 2.2, 0.5, 2.5, 3.3, 10.0 µM, respectively. Pluripotin (SC-1) decreases cell growth for 7 colon tumor cell lines. After a five-day exposure to 0.1 µM SC-1, the seven colon tumor lines are evaluated for changes in cell number and viability. There is a statistically significant decrease in cell number but >95% in viability[5]. Pluripotin significantly promoted the proliferation of RLECs and increased the dividing cells with positive Ki67 staining at the concentrations lower than 400 nM. The colony-forming efficiency increased from 13.5% in the control cells to 26.4% in the 200 or 400 nM pluripotin-treated cells. The number of colonies of moderate size (600-900 μm) increased significantly in the presence of pluripotin (above 60.0% at 200 nM or 400 nM) compared with the untreated normal cells (18.6%), whereas the number of small-sized colonies (<600 μm) decreased from 79.5% for the control cells to lower than 35.0% at 200 nM or 400 nM pluripotin. Moreover, the cells treated with pluripotin stained negative with SA-β-gal, while the untreated cells showed visible positive staining[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.82mL

0.36mL

0.18mL

9.08mL

1.82mL

0.91mL

18.16mL

3.63mL

1.82mL
参考文献

[1]Lin CJ, Amano T, et al. Improved derivation efficiency and pluripotency of stem cells from the refractory inbred C57BL/6 mouse strain by small molecules. PLoS One. 2014 Sep 11;9(9):e106916.

[2]Chen S, Do JT, et al. Self-renewal of embryonic stem cells by a small molecule. Proc Natl Acad Sci US A. 2006 Nov 14;103(46):17266-71.

[3]Yohannes Mebratu,et al. How ERK1/2 activation controls cell proliferation and cell death: Is subcellular localization the answer? Cell Cycle. 2009. 8(8), 1168-75.

[4]Chen S, et al. Self-renewal of embryonic stem cells by a small molecule. Proc Natl Acad Sci U S A. 2006. 103(46), 17266-71.

[5]Mertins SD, et al. A small molecule (pluripotin) as a tool for studying cancer stem cell biology: proof of concept. PLoS One. 2013. 8(2), e57099.

[6]Haoyun Duan,et al. Pluripotin enhances the expansion of rabbit limbal epithelial stem/progenitor cells in vitro. Exp Eye Res. 2012. 100, 52-8.