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RK-33

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Chemical Structure| 1070773-09-9 同义名 : -
CAS号 : 1070773-09-9
货号 : A179326
分子式 : C23H20N6O3
纯度 : 98%
分子量 : 428.443
MDL号 : MFCD30489740
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(116.7 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
靶点

  • helicase
描述 RK-33 demonstrates the ability to inhibit a variety of cancer cell types, with IC50 values ranging between 3-6 µM. However, the PC3 cell line exhibits a much lower sensitivity to RK-33, with an IC50 exceeding 12 µM. Treatment with RK-33 leads to a notable increase in the G1 phase population in DU145 and LNCaP cell lines. In contrast, 22Rv1 cells show only a slight increase in the G1 phase upon RK-33 treatment, along with a significant decrease in the G2 phase population. Additionally, RK-33 induces a moderate elevation in the G1 phase in 22Rv1 cells[1].
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
A549 cells Cytotoxicity assay 72 h Cytotoxicity against human A549 cells after 72 hrs by WST1 assay, IC50=2.5 μM 21572541
A549 cells Cytotoxicity assay 72 h Cytotoxicity against human A549 cells after 72 hrs by WST1 assay, IC50=2.56 μM 23290252
H460 cells Cytotoxicity assay 72 h Cytotoxicity against human H460 cells after 72 hrs by WST1 assay, IC50=2.8 μM 21572541
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.33mL

0.47mL

0.23mL

11.67mL

2.33mL

1.17mL

23.34mL

4.67mL

2.33mL
参考文献

[1]Xie M,et al. RK-33 radiosensitizes prostate cancer cells by blocking the RNA helicase DDX3. Cancer Res. 2016 Sep 12.

[2]Bol GM, e tal. PLGA nanoparticle formulation of RK-33: an RNA helicase inhibitor against DDX3. Cancer Chemother Pharmacol. 2015 Oct;76(4):821-7.