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Calycosin

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Chemical Structure| 20575-57-9 同义名 : 异黄酮 ;Cyclosin;3'-Hydroxyformononetin
CAS号 : 20575-57-9
货号 : A178055
分子式 : C16H12O5
纯度 : 99%+
分子量 : 284.264
MDL号 : MFCD00210598
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(369.38 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Calycosin, a phytoestrogen, is a partial agonist of ER and a calcium channel blocker. It also shows prevention of reperfusion injury. Calycosin can be isolated and purified from the herb of Astragalus membranaceus Bge. var. mongholicus with anti-oxidative activity. Calycosin had obvious anti-proliferation effects on SKOV3 cells in a dose- and time-dependent manner. calycosin up-regulated the Bax/Bcl-2 ratio and cleaved caspase-3, cleaved caspase-9 expression in a dose-dependent manner[3]. In mice bearing MCF-7 or SKBR3 xenografts, tumor growth was inhibited by calycosin, and changes in expression the levels of WDR7-7 and GPR30 (G-protein coupled estrogen receptor 30) in tumor tissues were similar to those in cultured MCF-7 and SKBR3 cells[4]. Calycosin is more effective in inhibiting breast cancer growth in comparison with genistein, through its regulation of Akt signaling pathways and HOTAIR expression[5]. Calycosin inhibited oxidative stress and autophagy in CKD (chronic kidney disease) induced skeletal muscle atrophy and in TNF-α-induced C2C12 myotube atrophy, partially by regulating the AMPK/SKP2/CARM1 signalling pathway[6]. Calycosin dose-dependently inhibited cell viability and increased LDH release in CC (cervical cancer) cells, suggesting the cytotoxic effect of calycosin on CC cells. Calycosin enhanced the apoptotic rate and caspase-3 activity and decreased the number of invaded cells in CC cells. Calycosin inhibited viability, induced apoptosis, and suppressed invasion of CC cells by upregulating tumor suppressor miR-375[7].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.52mL

0.70mL

0.35mL

17.59mL

3.52mL

1.76mL

35.18mL

7.04mL

3.52mL
参考文献

[1]Chen J, Lin C, et al. Calycosin and genistein induce apoptosis by inactivation of HOTAIR/p-Akt signaling pathway in human breast cancer MCF-7 cells. Cell Physiol Biochem. 2015;35(2):722-8.

[2]Chen J, Liu L, et al. Calycosin promotes proliferation of estrogen receptor-positive cells via estrogen receptors and ERK1/2 activation in vitro and in vivo. Cancer Lett. 2011 Sep 28;308(2):144-51.

[3]Zhou Y, Liu QH, Liu CL, Lin L. Calycosin induces apoptosis in human ovarian cancer SKOV3 cells by activating caspases and Bcl-2 family proteins. Tumour Biol. 2015 Jul;36(7):5333-9

[4]Tian J, Wang Y, Zhang X, Ren Q, Li R, Huang Y, Lu H, Chen J. Calycosin inhibits the in vitro and in vivo growth of breast cancer cells through WDR7-7-GPR30 Signaling. J Exp Clin Cancer Res. 2017 Nov 2;36(1):153

[5]Chen J, Lin C, Yong W, Ye Y, Huang Z. Calycosin and genistein induce apoptosis by inactivation of HOTAIR/p-Akt signaling pathway in human breast cancer MCF-7 cells. Cell Physiol Biochem. 2015;35(2):722-8

[6]Hu R, Wang MQ, Liu LY, You HY, Wu XH, Liu YY, Wang YJ, Lu L, Xiao W, Wei LB. Calycosin inhibited autophagy and oxidative stress in chronic kidney disease skeletal muscle atrophy by regulating AMPK/SKP2/CARM1 signalling pathway. J Cell Mol Med. 2020 Oct;24(19):11084-11099

[7]Zhang D, Sun G, Peng L, Tian J, Zhang H. Calycosin inhibits viability, induces apoptosis, and suppresses invasion of cervical cancer cells by upregulating tumor suppressor miR-375. Arch Biochem Biophys. 2020 Sep 30;691:108478