产品说明书

ZM 336372

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Chemical Structure| 208260-29-1 同义名 : Zinc00581684
CAS号 : 208260-29-1
货号 : A175274
分子式 : C23H23N3O3
纯度 : 99%+
分子量 : 389.447
MDL号 : MFCD02683971
存储条件：

粉末 Keep in dark place,Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(128.39 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
靶点

  • C-Raf/Raf-1

    C-Raf, IC50:70 nM
描述 ZM 336372 is a potent inhibitor of human c-Raf, with an IC50 value of 0.07 μM in a standard assay containing 0.1 mM ATP. The IC50 value decreases to 0.01 μM at 0.025 mM ATP and increases to 0.9 μM at 2.5 mM ATP, indicating that ZM 336372 competitively inhibits with respect to ATP. Additionally, ZM 336372 is ten times more effective in inhibiting c-Raf compared to B-Raf[1]. Cell proliferation analysis using ZM336372 was conducted with the 3,4-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide growth assay on H727 and BON cells treated with control (DMSO) and 100 μM ZM336372 for up to 16 and 10 days, respectively. Both H727 and BON cell proliferation were inhibited in the presence of the drug compared to the controls. Specifically, H727 cells exhibited suppressed growth with ZM336372 treatment, with significantly less growth observed by day 6, continuing through day 16. Similarly, BON cells showed growth suppression starting from day 4, which persisted until day 10[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.57mL

0.51mL

0.26mL

12.84mL

2.57mL

1.28mL

25.68mL

5.14mL

2.57mL
参考文献

[1]Hall-Jackson CA, et al. Paradoxical activation of Raf by a novel Raf inhibitor. Chem Biol. 1999 Aug;6(8):559-68.

[2]Van Gompel JJ, et al. ZM336372, a Raf-1 activator, suppresses growth and neuroendocrine hormone levels in carcinoid tumor cells. Mol Cancer Ther. 2005 Jun;4(6):910-7.