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SRT 2104

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Chemical Structure| 1093403-33-8 同义名 : GSK2245840
CAS号 : 1093403-33-8
货号 : A173533
分子式 : C26H24N6O2S2
纯度 : 99%+
分子量 : 516.638
MDL号 : MFCD22572733
存储条件：

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 4 mg/mL(7.74 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Sirtuin1 (SIRT1) is an NAD+-dependent deacetylase involved in the regulation of calorie restriction pathways, which exert beneficial effects on insulin sensitivity and glucose homeostasis. SRT2104 is a synthetic activator of SIRT1. Treatment of C2C12 myoblasts with 3μM SRT2104 for 24 hours increased the protein expression of RelA/p65 compared to DMSO-treated controls. SRT2104 at the same dose also resulted in decreased acetylation of RelA/p65 in C2C12 myoblasts. The ability of SRT2104 to stimulate alkaline phosphatase activity in C2C12 cells was SIRT1 expression-dependent. The proliferation rate of C2C12 myoblasts was remarkably reduced by SRT2104 treatment (3μM). The supplementation of SRT2104 (100mg/kg) in standard AIN-93G diet significantly improved the survival of C57BL/6J mice with an increase in maximum lifespan of 4.9% and in mean lifespan of 9.7%. SRT2104-treated mice also showed better endurance performance on the treadmill and improved motor skills. The trabecular bone mineral density, trabecular connectivity, and trabecular bone volume were effectively improved in SRT2104-treated mice as compared to the control animals. SRT2104 supplementation also significantly decreased the serum levels of TNF-α (6.1 ± 0.7 vs. 3.9 ± 0.5pg/mL) and MCP-1 (72.0 ± 8.9 vs. 47.9 ± 8.9pg/mL) compared to the controls[3]. 100mg/kg of SRT2104 added to the diet significantly attenuated diabetes mellitus-induced aortic oxidative stress and inflammation, as 3-NT, 4-HNE, as well as the inflammatory markers VCAM-1 and ICAM-1 in the aortas of the diabetic mice were decreased by SRT2104[4].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.94mL

0.39mL

0.19mL

9.68mL

1.94mL

0.97mL

19.36mL

3.87mL

1.94mL
参考文献

[1]Mercken EM, Mitchell SJ, et al. SRT2104 extends survival of male mice on a standard diet and preserves bone and muscle mass. Aging Cell. 2014Oct;13(5):787-96.

[2]Jiang M, Zheng J, et al. Sirtuin 1 activator SRT2104 protects Huntington's disease mice. Ann Clin Transl Neurol. 2014 Dec;1(12):1047-52.

[3]Mercken EM, Mitchell SJ, Martin-Montalvo A, Minor RK, Almeida M, Gomes AP, Scheibye-Knudsen M, Palacios HH, Licata JJ, Zhang Y, Becker KG, Khraiwesh H, González-Reyes JA, Villalba JM, Baur JA, Elliott P, Westphal C, Vlasuk GP, Ellis JL, Sinclair DA, Bernier M, de Cabo R. SRT2104 extends survival of male mice on a standard diet and preserves bone and muscle mass. Aging Cell. 2014 Oct;13(5):787-96.

[4]Wu H, Wu J, Zhou S, Huang W, Li Y, Zhang H, Wang J, Jia Y. SRT2104 attenuates diabetes-induced aortic endothelial dysfunction via inhibition of P53. J Endocrinol. 2018 Apr;237(1):1-14.