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Puromycin aminonucleoside

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Chemical Structure| 58-60-6 同义名 : 维生素E醋酸酯 ;NSC 3056;PANS;SAN;ARDMA;Stylomycin aminonucleoside;PAN
CAS号 : 58-60-6
货号 : A170018
分子式 : C12H18N6O3
纯度 : 98%
分子量 : 294.31
MDL号 : MFCD00063462
存储条件：

粉末 Keep in dark place,Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 25 mg/mL(84.94 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 40 mg/mL(135.91 mM)，配合低频超声，并水浴加热至45℃助溶
动物实验配方：
生物活性
描述 Puromycin aminonucleoside is the aminonucleoside portion of the antibiotic puromycin, and used in nephrosis animal models[3]. Puromycin aminonucleoside (30 μg/mL) markedly increases p53 protein levels in podocytes. Puromycin aminonucleoside-induced podocyte apoptosis is p53 dependent and time-dependent[4]. PAN (Puromycin Aminonucleoside) treatment increased ER (endoplasmic reticulum) stress markers such as activating transcription factor (ATF) 6α and caspase-12 in a dose-dependent manner at 12 and 24 hours, respectively. PAN-induced ER stress increased oxidative stress and subsequently induced apoptosis, and could be mitigated by inhibition of PI3-kinase signaling[5]. Rats given Puromycin aminonucleoside (100 mg/kg, s.c.) gain less weight and their serum creatinine levels are higher than the control rats. The pathogenesis of PAN toxicity is closely related to adenosine metabolism and that ADA (adenosine deaminase) plays a key role in this model[6]. In PAN-treated MCs (mesangial cells), ERRα (estrogen-related receptor-α) overexpression further aggravated PAN-induced apoptosis[7].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.40mL

0.68mL

0.34mL

16.99mL

3.40mL

1.70mL

33.98mL

6.80mL

3.40mL
参考文献

[1]Xia L, Zhou M, et al. Podocyte-specific expression of organic cation transporter PMAT: implication in puromycin aminonucleoside nephrotoxicity. Am J Physiol Renal Physiol. 2009 Jun;296(6):F1307-13.

[2]Wada T, Pippin JW, et al. Dexamethasone prevents podocyte apoptosis induced by puromycin aminonucleoside: role of p53 and Bcl-2-related family proteins. J Am Soc Nephrol. 2005 Sep;16(9):2615-25.

[3]Lacalle RA, Tercero JA, Jiménez A. Cloning of the complete biosynthetic gene cluster for an aminonucleoside antibiotic, puromycin, and its regulated expression in heterologous hosts. EMBO J. 1992 Feb;11(2):785-92

[4]Wada T, Pippin JW, Marshall CB, Griffin SV, Shankland SJ. Dexamethasone prevents podocyte apoptosis induced by puromycin aminonucleoside: role of p53 and Bcl-2-related family proteins. J Am Soc Nephrol. 2005 Sep;16(9):2615-25

[5]Min SY, Ha DS, Ha TS. Puromycin aminonucleoside triggers apoptosis in podocytes by inducing endoplasmic reticulum stress. Kidney Res Clin Pract. 2018 Sep;37(3):210-221

[6]Nosaka K, Takahashi T, Nishi T, Imaki H, Suzuki T, Suzuki K, Kurokawa K, Endou H. An adenosine deaminase inhibitor prevents puromycin aminonucleoside nephrotoxicity. Free Radic Biol Med. 1997;22(4):597-605

[7]Gong W, Song J, Chen X, Li S, Yu J, Xia W, Ding G, Zhang Y, Jia Z, Zhang A, Huang S. Estrogen-related receptor-α mediates puromycin aminonucleoside-induced mesangial cell apoptosis and inflammatory injury. Am J Physiol Renal Physiol. 2019 May 1;316(5):F906-F913