产品说明书
Nα-Tosyl-L-arginine Methyl Ester HCl
 |
同义名 : | TAME hydrochloride;N-4-Tosyl-L-arginine methyl ester (hydrochloride);Tosylarginine methyl ester;TAME |
| CAS号 : | 1784-03-8 | |
| 货号 : | A169128 | |
| 分子式 : | C14H23ClN4O4S | |
| 纯度 : | 98% | |
| 分子量 : | 378.875 | |
| MDL号 : | MFCD00012578 | |
| 存储条件： |
粉末 Inert atmosphere,Room Temperature 液体 -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 150 mg/mL(395.91 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO H2O: 100 mg/mL(263.94 mM) |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | The anaphase-promoting complex/cyclosome (APC/C) is a large multi-subunit complex that functions as a RING domain E3 ubiquitin ligase to regulate transitions through the cell cycle, achieved by controlling the defined ubiquitin-dependent degradation of specific cell cycle regulators[1]. Tos-Arg-OMe HCl (TAME) is a kind of amino acid derivatives, is a substrate for trypsin, plasmin, thrombin, and other proteases. It is also an inhibitor of anaphase-promoting complex/cyclosome (APC/C or APC), which binds to APC/C and prevents its activation by Cdc20 and Cdh1, produces mitotic arrest[2]. Cell surface Tos-Arg-OMe esterase activity was inhibited by aprotinin, benzamidine, pentamidine, and a tris-amidine derivative (alpha,alpha',alpha''-tris(3-amidinophenoxy)mesitylene)[3]. Acetone-kaolin activation of plasma produced the expected 30-fold increase in Tos-Arg-OMe esterase activity but no change in immunoreactive tissue kallikrein levels[4]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
2.64mL 0.53mL 0.26mL |
13.20mL 2.64mL 1.32mL |
26.39mL 5.28mL 2.64mL |
| 参考文献 |
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