产品说明书

IDO5L

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Chemical Structure| 914471-09-3 同义名 : INCB024360 analog;Epacadostat analogue;INCB14943
CAS号 : 914471-09-3
货号 : A165598
分子式 : C9H7ClFN5O2
纯度 : 98%
分子量 : 271.636
MDL号 : -
存储条件：

粉末 Keep in dark place,Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(184.07 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：

IP 2% DMSO+2% Tween80+40% PEG300+water 5 mg/mL clear

PO 0.5% CMC-Na 30 mg/mL suspension

生物活性
描述 IDO5L is a potent inhibitor of IDO with an IC50 value of 19 nM in Hela cells[1].
作用机制 INCB024360 Analogue is a reversible and competitive inhibitor of IDO1, which can bound to IDO through the oxygen of the hydroxyamidine to the iron of the heme in the ferrous state.
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
HEK293 clone 6 cells Function assay Inhibition of human IDO1 transfected in HEK293 clone 6 cells by HPLC analysis 22616902
human HeLa cells Function assay Inhibition of indoleamine 2,3-dioxygenase in IFN-gamma-stimulated human HeLa cells assessed as kynurenine formation by spectrophotometry, IC50=19 nM 19507862
mouse B16 cells Function assay Inhibition of indoleamine 2,3-dioxygenase in mouse B16 cells assessed as kynurenine formation by spectrophotometry, IC50=46 nM 19507862
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.68mL

0.74mL

0.37mL

18.41mL

3.68mL

1.84mL

36.81mL

7.36mL

3.68mL
参考文献

[1]Yue EW, et al. Discovery of potent competitive inhibitors of indoleamine 2,3-dioxygenase with in vivo pharmacodynamic activity and efficacy in a mouse melanoma model. J Med Chem. 2009 Dec 10;52(23):7364-7.